Luo Yanni, Li Caiying, Ye Fanggui, Tian Jianniao, Tan Xuecai, Hu Shengqiang, Zhao Shulin
State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, School of Chemistry and Chemical and Engineering, Guangxi University for Nationalities, Nanning 530008, China.
Anal Chem. 2024 Apr 23;96(16):6483-6492. doi: 10.1021/acs.analchem.4c00889. Epub 2024 Apr 13.
A disease-targeting nanoplatform that integrates imaging with therapeutic activity would facilitate early diagnosis, treatment, and therapeutic monitoring. To this end, a macrophage membrane-coated Cu-WO-Hydro820 (CWHM) nanoreactor was prepared. This reactor was shown to target inflammatory tissues. The reactive oxygen species (ROS) such as HO and ·OH in inflammatory tissues can react with Hydro820 in the reactor to form the NIR fluorophore IR820. This process allowed photoacoustic/fluorescence dual-mode imaging of HO and ·OH, and it is expected to permit visual diagnosis of inflammatory diseases. The Cu-WO nanoparticles within the nanoreactor shown catalase and superoxide enzyme mimetic activity, allowing the nanoreactor to catalyze the decomposition of HO and ·O in inflammatory cells of hepatic tissues in a mouse model of liver injury, thus alleviating the oxidative stress of damaged liver tissue. This nanoreactor illustrates a new strategy for the diagnosis and treatment of hepatitis and inflammatory liver injury.
一种将成像与治疗活性相结合的疾病靶向纳米平台将有助于早期诊断、治疗和治疗监测。为此，制备了一种巨噬细胞膜包覆的Cu-WO-Hydro820（CWHM）纳米反应器。该反应器显示出对炎症组织的靶向性。炎症组织中的活性氧（ROS）如HO和·OH可与反应器中的Hydro820反应形成近红外荧光团IR820。这一过程实现了对HO和·OH的光声/荧光双模态成像，有望实现炎症性疾病的可视化诊断。纳米反应器内的Cu-WO纳米颗粒表现出过氧化氢酶和超氧化物酶模拟活性，使纳米反应器能够催化肝损伤小鼠模型肝组织炎症细胞中HO和·O的分解，从而减轻受损肝组织的氧化应激。这种纳米反应器为肝炎和炎症性肝损伤的诊断和治疗说明了一种新策略。
