Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625 021, Tamilnadu, India.
Department of Chemistry, Vivekananda College, Tiruvedakam West, Madurai 625 234, Tamilnadu, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jul 5;315:124271. doi: 10.1016/j.saa.2024.124271. Epub 2024 Apr 8.
As an important biomarker for renal related diseases, detection of urea is playing a vital role in human biofluids on clinical diagnosis concern. In this work, a synthetic salicyaldehyde based imine fluorophore was synthesized using sonication method and conjugated with urease which was used as fluorescent biosensor for the detection of urea in serum samples. This enzyme based biosensor has shown a good selectivity and sensitivity towards urea with the linear range from 2 to 80 mM and the detection limit of 73 µM. The sensing response obtain is highly agreeing with existing analytical technique for urea detection which strongly recommends this biosensor for clinical application.
作为与肾脏相关疾病的重要生物标志物，在临床诊断关注的人体生物流体中，对尿素的检测发挥着至关重要的作用。在这项工作中，使用超声法合成了一种基于水杨醛的亚胺荧光团，并将其与脲酶偶联，用作荧光生物传感器，用于检测血清样品中的尿素。这种基于酶的生物传感器对尿素具有良好的选择性和灵敏度，线性范围为 2 至 80 mM，检测限为 73 µM。获得的传感响应与现有的尿素检测分析技术高度吻合，强烈推荐该生物传感器用于临床应用。
