Wahab Walaa A Abdel
Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Cairo, Egypt.
Microb Cell Fact. 2025 Jul 18;24(1):167. doi: 10.1186/s12934-025-02791-0.
Enzyme stabilization is one of the most critical steps help in applying the enzymes on industrial scale efficiently. Enzymes have use in a variety of areas, including medical, industry, food, and even beauty and cosmetics. The industrial application of enzymes is constantly limited by stability and cost. Enzyme synthesis on a large scale involves multiple steps, therefore stability and repeatability are critical. These two goals are crucial on an industrial scale because they translate to reduced time, effort, and cost. Enzyme stabilization provides the stability and reusability required for successful application. Immobilization with appropriate carriers and conjugation with chemically modified polysaccharides are the most common and low-cost strategies used for enzyme stabilization. These tactics enhance the enzyme's physicochemical characteristics, making it better suited for industrial applications that benefit our daily lives. This review is an attempt to provide a spot on each method, their progress, benefits and draw backs, and their application.
酶的稳定化是有助于在工业规模上高效应用酶的最关键步骤之一。酶在包括医学、工业、食品乃至美容和化妆品在内的各种领域都有应用。酶的工业应用一直受到稳定性和成本的限制。大规模酶合成涉及多个步骤，因此稳定性和可重复性至关重要。这两个目标在工业规模上至关重要，因为它们意味着时间、精力和成本的减少。酶的稳定化提供了成功应用所需的稳定性和可重复使用性。用合适的载体固定化以及与化学修饰的多糖结合是用于酶稳定化的最常见且低成本的策略。这些策略增强了酶的物理化学特性，使其更适合有益于我们日常生活的工业应用。本综述旨在对每种方法、它们的进展、优点和缺点以及它们的应用进行详细阐述。
