Ogaya Yuko, Kadota Tamami, Hamada Masakazu, Nomura Ryota, Nakano Kazuhiko
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan.
Department of Oral & Maxillofacial Oncology and Surgery, Osaka University Graduate School of Dentistry, Osaka, Japan.
J Oral Microbiol. 2024 Apr 11;16(1):2339158. doi: 10.1080/20002297.2024.2339158. eCollection 2024.
infection is acquired in childhood via the oral cavity, although its relationship with the characteristics of the oral microbiome has not been elucidated. In this study, we performed comprehensive analysis of the oral microbiome in children and adults with or without H. pylori in the oral cavity.
Bacterial DNA was extracted from 41 adult and 21 child saliva specimens, and H. pylori was detected using PCR. 16S rRNA gene amplification was performed for next-generation sequencing. Bioinformatic analyses were conducted using Quantitative Insights into Microbial Ecology 2 (QIIME 2).
Faith's phylogenetic diversity analysis showed a significant difference between H. pylori-negative adult and child specimens in terms of α-diversity ( < 0.05), while no significant difference was observed between H. pylori-positive adult and child specimens. There was also a significant difference in β-diversity between H. pylori-positive and negative child specimens ( < 0.05). Taxonomic analysis at the genus level revealed that Porphyromonas was the only bacterium that was significantly more abundant in both H. pylori-positive adults and children than in corresponding negative specimens ( < 0.01 and < 0.05, respectively).
These results suggest unique oral microbiome characteristics in children with H. pylori infection in the oral cavity.
感染是在儿童期通过口腔获得的，尽管其与口腔微生物群特征的关系尚未阐明。在本研究中，我们对口腔中有或无幽门螺杆菌的儿童和成人的口腔微生物群进行了综合分析。
从41份成人和21份儿童唾液标本中提取细菌DNA，并使用PCR检测幽门螺杆菌。进行16S rRNA基因扩增以进行下一代测序。使用微生物生态学定量见解2（QIIME 2）进行生物信息学分析。
Faith系统发育多样性分析显示，幽门螺杆菌阴性的成人和儿童标本在α多样性方面存在显著差异（<0.05），而幽门螺杆菌阳性的成人和儿童标本之间未观察到显著差异。幽门螺杆菌阳性和阴性儿童标本在β多样性方面也存在显著差异（<0.05）。属水平的分类分析表明，卟啉单胞菌是唯一在幽门螺杆菌阳性的成人和儿童中均比相应阴性标本中显著更丰富的细菌（分别<0.01和<0.05）。
这些结果表明口腔中感染幽门螺杆菌的儿童具有独特的口腔微生物群特征。
