Koshy Divya S, Allardyce Benjamin J, Dumée Ludovic F, Sutti Alessandra, Rajkhowa Rangam, Agrawal Ruchi
TERI-Deakin Nanobiotechnology Centre, Sustainable Agriculture Division, The Energy and Resources Institute, TERI Gram, Gwal Pahari, Gurugram, Haryana 122001, India.
Institute for Frontier Materials, Deakin University, Geelong Waurn Ponds Campus, Pigdons Road, Geelong, VIC 3216, Australia.
ACS Omega. 2024 Mar 25;9(14):15768-15780. doi: 10.1021/acsomega.3c03367. eCollection 2024 Apr 9.
Hybrid protein-copper nanoflowers have emerged as promising materials with diverse applications in biocatalysis, biosensing, and bioremediation. Sericin, a waste biopolymer from the textile industry, has shown potential for fabricating such nanoflowers. However, the influence of the molecular weight of sericin on nanoflower morphology and peroxidase-like activity remains unexplored. This work focused on the self-assembly of nanoflowers using high- and low-molecular-weight (HMW and LMW) silk sericin combined with copper(II) as an inorganic moiety. The peroxidase-like activity of the resulting nanoflowers was evaluated using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydrogen peroxide (HO). The findings revealed that high-molecular-weight sericin hybrid nanoflowers (HMW-ShNFs) exhibited significantly higher peroxidase-like activity than low-molecular-weight sericin hybrid nanoflowers (LMW-ShNFs). Furthermore, HMW-ShNFs demonstrated superior reusability and storage stability, thereby enhancing their potential for practical use. This study also explored the application of HMW-ShNF for ciprofloxacin degradation to address the environmental and health hazards posed by this antibiotic in water. The results indicated that HMW-ShNFs facilitated the degradation of ciprofloxacin, achieving a maximum degradation of 33.2 ± 1% at pH 8 and 35 °C after 72 h. Overall, the enhanced peroxidase-like activity and successful application in ciprofloxacin degradation underscore the potential of HMW-ShNFs for a sustainable and ecofriendly remediation process. These findings open avenues for the further exploration and utilization of hybrid nanoflowers in various environmental applications.
杂合蛋白-铜纳米花已成为在生物催化、生物传感和生物修复等领域具有多种应用前景的材料。丝胶蛋白是纺织工业的一种废弃生物聚合物，已显示出制备此类纳米花的潜力。然而，丝胶蛋白分子量对纳米花形态和过氧化物酶样活性的影响仍未得到探索。这项工作聚焦于使用高分子量和低分子量（HMW和LMW）丝胶蛋白与作为无机部分的铜（II）自组装纳米花。使用2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和过氧化氢（HO）评估所得纳米花的过氧化物酶样活性。研究结果表明，高分子量丝胶蛋白杂合纳米花（HMW-ShNFs）表现出比低分子量丝胶蛋白杂合纳米花（LMW-ShNFs）显著更高的过氧化物酶样活性。此外，HMW-ShNFs表现出优异的可重复使用性和储存稳定性，从而增强了其实际应用潜力。本研究还探索了HMW-ShNF用于环丙沙星降解，以解决这种抗生素在水中对环境和健康造成的危害。结果表明，HMW-ShNFs促进了环丙沙星的降解，在pH 8和35°C下72小时后最大降解率达到33.2±1%。总体而言，增强的过氧化物酶样活性以及在环丙沙星降解中的成功应用凸显了HMW-ShNFs在可持续和生态友好修复过程中的潜力。这些发现为进一步探索和利用杂合纳米花在各种环境应用中开辟了道路。
