Department of BioNano Technology, Gachon University, Gyeonggi, 13120, South Korea.
Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, South Korea.
Adv Healthc Mater. 2019 May;8(9):e1801507. doi: 10.1002/adhm.201801507. Epub 2019 Mar 7.
It is reported that glucose oxidase (GOx)-copper hybrid nanoflowers embedded with Fe O magnetic nanoparticles (MNPs) exhibit superior peroxidase-mimicking activity as well as substrate channeling for glucose detection. This is due to the synergistic integration of GOx, crystalline copper phosphates and MNPs being in close proximity within the nanoflowers. The preparation of MNP-embedded GOx-copper hybrid nanoflowers (MNPs-GOx NFs) begins with the facile conjugation of amine-functionalized MNPs with GOx molecules via electrostatic attraction, followed by the addition of copper sulfate that leads to full blooming of the hybrid nanoflowers. In the presence of glucose, the catalytic action of GOx entrapped in the nanoflowers generates H O , which is subsequently used by peroxidase-mimicking MNPs and copper phosphate crystals, located close to GOx molecules, to convert Amplex UltraRed substrate into a highly fluorescent product. Using this strategy, the target glucose is successfully determined with excellent selectivity, stability, and magnetic reusability. This biosensor based on hybrid nanoflowers also exhibits a high degree of precision and reproducibility when applied to real human blood samples. Such novel MNP-embedded enzyme-inorganic hybrid nanoflowers have a great potential to be expanded to any oxidases, which will be highly beneficial for the detection of various other clinically important target molecules.
据报道，葡萄糖氧化酶（GOx）-铜杂化纳米花嵌入 Fe3O4磁性纳米颗粒（MNPs）表现出优异的过氧化物酶模拟活性以及葡萄糖检测的底物通道化。这是由于 GOx、结晶铜磷酸盐和 MNPs 之间在纳米花内紧密结合的协同集成。MNP 嵌入 GOx-铜杂化纳米花（MNPs-GOx NFs）的制备始于通过静电吸引将胺功能化的 MNPs 与 GOx 分子进行简单的共轭，然后加入硫酸铜，导致杂化纳米花完全绽放。在葡萄糖存在下，纳米花内包裹的 GOx 的催化作用产生 H2O2，随后被靠近 GOx 分子的过氧化物酶模拟 MNPs 和铜磷酸盐晶体利用，将 Amplex UltraRed 底物转化为高荧光产物。使用这种策略，可以成功地确定目标葡萄糖，具有优异的选择性、稳定性和磁性可重复使用性。当应用于真实的人血样本时，基于杂化纳米花的这种生物传感器还表现出高度的精度和重现性。这种新型的 MNP 嵌入酶-无机杂化纳米花具有扩展到任何氧化酶的巨大潜力，这将非常有利于检测各种其他临床重要的目标分子。
