Zeraatiannejad Mastaneh, Mokhtari Mohammad Javad, Borhani-Haghighi Afshin
Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran.
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Rep Biochem Mol Biol. 2023 Oct;12(3):476-486. doi: 10.61186/rbmb.12.3.476.
Fast diagnosing ischemic stroke (IS) is a critical issue in clinical studies, as it allows more effective therapy and stops the progression of IS. The blood level of circular RNAs (CircRNAs) after stroke may be a rapid diagnostic marker.
In this study, the blood level of circRNAs was evaluated using a real-time polymerase chain reaction (PCR). We used logistic and linear regression analysis to assess the potential of circRNAs levels with the risk of IS.
circRNA DLG associated protein 4 (CircDLGAP4) was decreased in patients compared with controls, and logistic regression showed its expression negatively associated with IS risk. The expression level of human genome version 38_Circular_0008980 (hg38_circ_0008980) was reduced significantly in patients with small vessel disease (SVD), and the linear regression analysis showed a negative relationship between hg38_circ_0008980 expressions with SVD subtype. hg38_circ_0008980 expression relative to controls showed a significant association with IS risk.
Taken together, we found a significant decrease in the level of hg38_circ_0008980 after IS; it may act as a novel circRNA in IS pathophysiology with a positive correlation with stroke severity.
快速诊断缺血性中风（IS）是临床研究中的关键问题，因为它能实现更有效的治疗并阻止IS进展。中风后环状RNA（CircRNAs）的血液水平可能是一种快速诊断标志物。
在本研究中，使用实时聚合酶链反应（PCR）评估CircRNAs的血液水平。我们使用逻辑回归和线性回归分析来评估CircRNAs水平与IS风险的相关性。
与对照组相比，患者体内环状RNA盘状结构域蛋白4（CircDLGAP4）水平降低，逻辑回归显示其表达与IS风险呈负相关。在小血管病（SVD）患者中，人类基因组版本38_环状_0008980（hg38_circ_0008980）的表达水平显著降低，线性回归分析显示hg38_circ_0008980表达与SVD亚型之间呈负相关。相对于对照组，hg38_circ_0008980表达与IS风险显著相关。
综上所述，我们发现IS后hg38_circ_0008980水平显著降低；它可能是IS病理生理学中的一种新型环状RNA，与中风严重程度呈正相关。
