Eletr Loai F, Ibnouf Sahar H, Salih Tanzeel A, Ibrahim Hadba I, Mustafa Mustafa I, Alhashmi Nasma A, Alfaki Mohamed
Computing and Bioinformatics, Faculty of Science, Port Said University, Port Said, EGY.
Pharmacy, Omdurman Islamic University, Khartoum, SDN.
Cureus. 2024 Mar 14;16(3):e56171. doi: 10.7759/cureus.56171. eCollection 2024 Mar.
Deoxyribonuclease 1 (DNASE1) is an important gene associated with several cancers, including liver, bladder, and gastric cancer. It has been linked to autoimmune illnesses, including systemic lupus erythematosus, which may lead to cancer formation. However, the role of DNASE1 in cancer has not been studied.
We performed a pan-cancer analysis using bioinformatics tools, including Tumor Immune Estimation Resource (TIMER), Gene Expression Profiling Interactive Analysis (GEPIA), and University of Alabama at Birmingham Cancer Data Analysis Portal (UALCAN) databases, Kaplan-Meier plotter, and cBioPortal, to investigate the expression of DNASE1 across various cancers as well as its association with immune infiltration and genetic alterations. Public datasets were used to validate DNASE1 expression in kidney renal clear cell carcinoma (KIRC) and kidney papillary renal cell carcinoma (KIRP) samples.
DNASE1 was found to be highly expressed in many cancers, such as bladder urothelial carcinoma (BLCA), breast invasive carcinoma (BRCA), head and neck squamous cell carcinoma (HNSC), and was lowly expressed in other cancers, including KIRC, KIRP, and thyroid carcinoma (THCA). Additionally, TIMER results showed an association of DNASE1 with immune cell infiltration in KIRC and KIRP. Survival analysis indicated that high DNASE1 expression was associated with poor prognosis in KIRC. We also discovered that altered DNASE1 expression was related to poor prognosis in The Cancer Genome Atlas (TCGA) tumors.
DNASE1 could potentially be used as a prognostic and diagnostic biomarker for KIRC and as a diagnostic biomarker for KIRP.
脱氧核糖核酸酶1（DNASE1）是一种与多种癌症相关的重要基因，包括肝癌、膀胱癌和胃癌。它还与自身免疫性疾病有关，如系统性红斑狼疮，这可能导致癌症形成。然而，DNASE1在癌症中的作用尚未得到研究。
我们使用生物信息学工具进行了泛癌分析，包括肿瘤免疫评估资源（TIMER）、基因表达谱交互式分析（GEPIA）、阿拉巴马大学伯明翰分校癌症数据分析门户（UALCAN）数据库、Kaplan-Meier绘图仪和cBioPortal，以研究DNASE1在各种癌症中的表达及其与免疫浸润和基因改变的关联。使用公共数据集验证DNASE1在肾透明细胞癌（KIRC）和肾乳头状肾细胞癌（KIRP）样本中的表达。
发现DNASE1在许多癌症中高表达，如膀胱尿路上皮癌（BLCA）、乳腺浸润性癌（BRCA）、头颈部鳞状细胞癌（HNSC），而在其他癌症中低表达，包括KIRC、KIRP和甲状腺癌（THCA）。此外，TIMER结果显示DNASE1与KIRC和KIRP中的免疫细胞浸润有关。生存分析表明，DNASE1高表达与KIRC的预后不良相关。我们还发现，DNASE1表达改变与癌症基因组图谱（TCGA）肿瘤的预后不良有关。
DNASE1可能潜在地用作KIRC的预后和诊断生物标志物以及KIRP的诊断生物标志物。
