Herrmann D B
J Natl Cancer Inst. 1985 Sep;75(3):423-30.
Susceptibility of eight different cell types of murine or human origins to alkyl lysophospholipid analogue (ALP)-induced cytolysis correlated well with a selective, dose-dependent inhibition of radiolabeled oleic acid incorporation into phosphatidylcholine (PC) and a concomitant stimulation of incorporation into neutral lipids (NL), mainly triacylglycerols. In resistant cells (murine macrophages, L929S, K562, and rMeth A) a counts per minute NL/counts per minute PC ratio of 0.8-1.0 was observed with 30 micrograms ALP/ml; in sensitive tumor targets (Meth A, HL60, YAC, and ABLS-8.1) values greater than 2.7 were found with 5-10 micrograms ALP/ml. Changes in lipid metabolism preceded cytolysis in Meth A fibrosarcoma cells. In degradation experiments the percentage of total lipid radioactivity in PC was reduced after 24 hours to 47% compared to that in controls in sensitive Meth A with 10 micrograms ALP/ml. The macrophage-PC was unaffected at the same concentration. Sensitivity to ALP was independent of cell proliferation. Resistance was not restricted to normal cells and was inducible in Meth A (and rMeth A).
八种不同的鼠源或人源细胞类型对烷基溶血磷脂类似物（ALP）诱导的细胞溶解的敏感性，与放射性标记的油酸掺入磷脂酰胆碱（PC）的选择性、剂量依赖性抑制以及同时刺激其掺入中性脂质（NL）（主要是三酰甘油）密切相关。在抗性细胞（鼠巨噬细胞、L929S、K562和rMeth A）中，当ALP浓度为30微克/毫升时，观察到每分钟NL计数/每分钟PC计数的比值为0.8 - 1.0；在敏感肿瘤靶细胞（Meth A、HL60、YAC和ABLS - 8.1）中，当ALP浓度为5 - 10微克/毫升时，该比值大于2.7。在Meth A纤维肉瘤细胞中，脂质代谢变化先于细胞溶解。在降解实验中，对于敏感的Meth A细胞，当ALP浓度为10微克/毫升时，24小时后PC中总脂质放射性的百分比降至47%，而对照细胞为对照组。相同浓度下巨噬细胞的PC不受影响。对ALP的敏感性与细胞增殖无关。抗性不仅限于正常细胞，在Meth A（和rMeth A）中可诱导产生。
