Hillbertz-Nilsson K, Forsberg J G
J Natl Cancer Inst. 1985 Sep;75(3):575-80.
Uterine cervical fibroblasts and kidney cells from 3-to 4-day-old, previously untreated or phenobarbital [(PBA) CAS: 50-06-6]-treated female NMRI mice were cultured in vitro in the presence of diethylstilbestrol [(DES) CAS: 56-53-1] or 17 beta-estradiol [(E2) CAS: 50-28-2]. DES at 10(-7) M in the culture medium increased the number of sister chromatid exchanges (SCE); no further increases was obtained with higher concentrations of DES. Pretreatment of the females with PBA resulted in an increase in SCE when DES was used in the range of 10(-7) to 10(-5) M. The level of this increase was significantly higher than that in fibroblasts from untreated females. E2 had no effect. DES had no effect on SCE in kidney cells from PBA-treated females. Indomethacin and alpha-naphthoflavone reduced the number of SCE in PBA-exposed fibroblasts to an intermediate level. The presence of both enzyme inhibitors in the medium depressed the number of SCE to the control level.
从3至4日龄、未经处理或经苯巴比妥[（PBA）化学物质登记号：50 - 06 - 6]处理的雌性NMRI小鼠中获取子宫颈成纤维细胞和肾细胞，在体外于己烯雌酚[（DES）化学物质登记号：56 - 53 - 1]或17β - 雌二醇[（E2）化学物质登记号：50 - 28 - 2]存在的情况下进行培养。培养基中10⁻⁷M的DES增加了姐妹染色单体交换（SCE）的数量；更高浓度的DES未导致进一步增加。当在10⁻⁷至10⁻⁵M范围内使用DES时，用PBA对雌性小鼠进行预处理会导致SCE增加。这种增加的水平显著高于未处理雌性小鼠的成纤维细胞中的水平。E2没有影响。DES对经PBA处理的雌性小鼠的肾细胞中的SCE没有影响。吲哚美辛和α - 萘黄酮将PBA暴露的成纤维细胞中的SCE数量降低至中间水平。培养基中同时存在这两种酶抑制剂会将SCE数量降低至对照水平。
