Shammeri Ali, Abu-Huwaij Rana, Hamed Rania
Pharmacological and Diagnostic Research Center, Faculty of Pharmacy, Al-Ahliyya Amman University, Al-Salt, Jordan.
College of Pharmacy, Amman Arab University, Amman, Jordan.
Pharm Dev Technol. 2024 Apr;29(4):383-392. doi: 10.1080/10837450.2024.2341244. Epub 2024 Apr 15.
A novel approach was devised to address the challenges in delivering cisplatin (CIS) for lung cancer treatment. This involved the development of a non-invasive hydrogel delivery system, aiming to minimize side effects associated with its administration. Using carbopol 971 (CP) and chitosan (CH) at varying ratios, the hydrogels were prepared and loaded with eco-friendly iron oxide nanoparticles (IONPs) conjugated to CIS. The physical properties, yield, drug loading, and cytotoxicity against lung cancer cell lines (A549) were assessed, along with hydrogel rheological properties and drug diffusion. Hydrogel A1 that composed of 1:1 of CP:CH hydrogel loaded with 100 mg IONPs and 250 µg CIS demonstrated distinctive properties that indicate its suitability for potential delivery. The loaded greenly synthesized IONPs@CIS exhibited a particle size of 23.0 nm, polydispersity index of 0.47, yield of 71.6%, with 88.28% drug loading. They displayed significant cytotoxicity (61.7%) against lung cancer cell lines (A549), surpassing free CIS cytotoxicity (28.1%). Moreover, they demonstrated shear-thinning behaviour, viscoelastic properties, and Fickian drug release profile over 24 h (flux 2.34 µg/cm/h, and permeability 0.31 cm/h).
设计了一种新方法来应对顺铂（CIS）用于肺癌治疗时面临的挑战。这涉及开发一种非侵入性水凝胶递送系统，旨在将其给药相关的副作用降至最低。使用不同比例的卡波姆971（CP）和壳聚糖（CH）制备水凝胶，并负载与CIS偶联的环保型氧化铁纳米颗粒（IONPs）。评估了水凝胶的物理性质、产率、载药量以及对肺癌细胞系（A549）的细胞毒性，同时还评估了水凝胶的流变学性质和药物扩散情况。由1:1的CP:CH水凝胶组成、负载100 mg IONPs和250 μg CIS的水凝胶A1表现出独特的性质，表明其适合潜在递送。负载的绿色合成IONPs@CIS的粒径为23.0 nm，多分散指数为0.47，产率为71.6%，载药量为88.28%。它们对肺癌细胞系（A549）显示出显著的细胞毒性（61.7%），超过了游离CIS的细胞毒性（28.1%）。此外，它们表现出剪切变稀行为、粘弹性性质以及在24小时内的菲克型药物释放曲线（通量为2.34 μg/cm/h，渗透率为0.31 cm/h）。
