State Engineering Technology Institute for Karst Desertfication Control, School of Karst Science, Guizhou Normal University, Guiyang 5500025, China.
Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550025, China.
J Anim Sci. 2024 Jan 3;102. doi: 10.1093/jas/skae107.
The present study aimed to investigate the effects of deoxynivalenol (DON) stimulation on inflammatory injury and the expression of the glucose transporters sodium-dependent glucose transporter 1 (SGLT1) and glucose transporter protein 2 (GLU2) in porcine small intestinal epithelial cells (IPEC-J2). Additionally, the study aimed to provide initial insights into the connection between the expression of glucose transporters and the inflammatory injury of IPEC-J2 cells. DON concentration and DON treatment time were determined using the CCK‑8 assay. Accordingly, 1.0 µg/mL DON and treatment for 24 h were chosen for subsequent experiments. Then IPEC-J2 cells were treated without DON (CON, N = 6) or with 1 μg/mL DON (DON, N = 6). Lactate dehydrogenase (LDH) content, apoptosis rate, and proinflammatory cytokines including interleukin (IL)-1β, Il-6, and tumor necrosis factor α (TNF-α) were measured. Additionally, the expression of AMP-activated protein kinase α1 (AMPK-α1), the content of glucose, intestinal alkaline phosphatase (AKP), and sodium/potassium-transporting adenosine triphosphatase (Na+/K+-ATPase) activity, and the expression of SGLT1 and GLU2 of IPEC-J2 cells were also analyzed. The results showed that DON exposure significantly increased LDH release and apoptosis rate of IPEC-J2 cells. Stimulation with DON resulted in significant cellular inflammatory damage, as evidenced by a significant increase in proinflammatory cytokines (IL-1β, IL-6, and TNF-α). Additionally, DON caused damage to the glucose absorption capacity of IPEC-J2 cells, indicated by decreased levels of glucose content, AKP activity, Na+/K+-ATPase activity, AMPK-α1 protein expression, and SGLT1 expression. Correlation analysis revealed that glucose absorption capacity was negatively correlated with cell inflammatory cytokines. Based on the findings of this study, it can be preliminarily concluded that the cell inflammatory damage caused by DON may be associated with decreased glucose absorption.
本研究旨在探讨脱氧雪腐镰刀菌烯醇（DON）刺激对猪小肠上皮细胞（IPEC-J2）炎症损伤及葡萄糖转运体钠依赖性葡萄糖转运蛋白 1（SGLT1）和葡萄糖转运蛋白 2（GLU2）表达的影响。此外，本研究还旨在初步探讨葡萄糖转运体的表达与 IPEC-J2 细胞炎症损伤之间的关系。CCK-8 法测定 DON 浓度和 DON 处理时间。因此，选择 1.0μg/mL DON 和 24 h 处理进行后续实验。然后用无 DON（CON，N=6）或 1μg/mL DON（DON，N=6）处理 IPEC-J2 细胞。测定乳酸脱氢酶（LDH）含量、细胞凋亡率和炎症因子白细胞介素（IL）-1β、IL-6 和肿瘤坏死因子α（TNF-α）。此外，还分析了 AMP 激活的蛋白激酶α1（AMPK-α1）的表达、葡萄糖含量、肠碱性磷酸酶（AKP）和钠/钾转运三磷酸腺苷酶（Na+/K+-ATPase）活性以及 IPEC-J2 细胞 SGLT1 和 GLU2 的表达。结果表明，DON 暴露显著增加了 IPEC-J2 细胞的 LDH 释放和细胞凋亡率。DON 刺激导致 IPEC-J2 细胞发生明显的细胞炎症损伤，表现为促炎细胞因子（IL-1β、IL-6 和 TNF-α）显著增加。此外，DON 还导致 IPEC-J2 细胞的葡萄糖吸收能力受损，表现为葡萄糖含量、AKP 活性、Na+/K+-ATPase 活性、AMPK-α1 蛋白表达和 SGLT1 表达降低。相关性分析表明，葡萄糖吸收能力与细胞炎症细胞因子呈负相关。基于本研究的结果，可以初步得出结论，DON 引起的细胞炎症损伤可能与葡萄糖吸收减少有关。
