Huang Gui-Fa, Luo Yu, Zhong Wen-Feng, Chen Qing-Yi, Deng Cheng-Cheng, Mo Ri-Jiang, Lin Wei-Xiong, Tian Qing-Qing
Guangdong E-fong Pharmaceutical Co., Ltd. Foshan 528244, China.
Hunan Yifang Tianjiang Pharmaceutical Co., Ltd. Changde 415000, China.
Zhongguo Zhong Yao Za Zhi. 2024 Mar;49(5):1217-1224. doi: 10.19540/j.cnki.cjcmm.20231212.102.
To investigate the quality differences between the seeds and husks of Amomum villosum and explore the rationality of using the seeds without husks, this study determined the content of protocatechuic acid, vanillic acid, epicatechin, quercitrin, volatile oil, water extract, and ethanol extract. The 2,2-diphenyl-1-picrylhydrazyl(DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), and hydroxyl radical scavenging activities were determined to evaluate the antioxidant activities of seeds and husks. The quality differences between the seeds and husks were assessed through orthogonal partial least squares-discriminant analysis(OPLS-DA) and analytic hierarchy process(AHP) combined with the entropy weight method(EWM). Significant differences(P<0.05) were observed in all 10 indicators between the seeds and husks. The levels of epicatechin, quercetin, and volatile oil were higher in the seeds, whereas those of protocatechuic acid, vanillic acid, water extract, and ethanol extract were higher in the husks. The seeds showed stronger scavenging ability against DPPH and ABTS radicals, while the husks showed a stronger scavenging effect on hydroxyl radicals. OPLS-DA significantly discriminated between the seeds and husks. Furthermore, volatile oil, water extract, DPPH radical scavenging rate, quercitrin, ABTS radical scavenging rate, hydroxyl radical scavenging rate, and vanillic acid were selected as the main differential indicators by variable importance in projection(VIP). Comprehensive scores calculated by AHP combined with EWM indicated that the seeds were superior to husks in terms of overall quality. However, there are still some dominant components and a certain antioxidant effect in the husks. Therefore, it is suggested to using Amomi Fructus with a certain amount of husks or utilizing the husks for other purposes.
为研究阳春砂种子与果壳的质量差异,探索去壳种子用药的合理性,本研究测定了原儿茶酸、香草酸、表儿茶素、槲皮苷、挥发油、水提取物和乙醇提取物的含量。通过测定2,2-二苯基-1-苦基肼自由基(DPPH)、2,2-联氮-二(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)和羟自由基清除活性来评价种子和果壳的抗氧化活性。通过正交偏最小二乘法判别分析(OPLS-DA)和层次分析法(AHP)结合熵权法(EWM)评估种子与果壳的质量差异。种子与果壳的10项指标均存在显著差异(P<0.05)。种子中表儿茶素、槲皮素和挥发油含量较高,而果壳中原儿茶酸、香草酸、水提取物和乙醇提取物含量较高。种子对DPPH和ABTS自由基的清除能力较强,而果壳对羟自由基的清除效果较强。OPLS-DA能显著区分种子与果壳。此外,通过变量投影重要性(VIP)筛选出挥发油、水提取物、DPPH自由基清除率、槲皮苷、ABTS自由基清除率、羟自由基清除率和香草酸作为主要差异指标。AHP结合EWM计算的综合得分表明,种子的整体质量优于果壳。然而,果壳中仍存在一些主要成分并具有一定的抗氧化作用。因此,建议使用带一定量果壳的阳春砂,或将果壳用于其他用途。