School of Modern Industry for Selenium Science and Engineering, National R&D Center for Se-Rich Agricultural Products Processing Technology, Wuhan Polytechnic University, Wuhan, 430023, China.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
Plant Cell Rep. 2024 Apr 15;43(5):116. doi: 10.1007/s00299-024-03204-z.
The study on the GmDWF1-deficient mutant dwf1 showed that GmDWF1 plays a crucial role in determining soybean plant height and yield by influencing the biosynthesis of brassinosteroids. Soybean has not adopted the Green Revolution, such as reduced height for increased planting density, which have proven beneficial for cereal crops. Our research identified the soybean genes GmDWF1a and GmDWF1b, homologous to Arabidopsis AtDWF1, and found that they are widely expressed, especially in leaves, and linked to the cellular transport system, predominantly within the endoplasmic reticulum and intracellular vesicles. These genes are essential for the synthesis of brassinosteroids (BR). Single mutants of GmDWF1a and GmDWF1b, as well as double mutants of both genes generated through CRISPR/Cas9 genome editing, exhibit a dwarf phenotype. The single-gene mutant exhibits moderate dwarfism, while the double mutant shows more pronounced dwarfism. Despite the reduced stature, all types of mutants preserve their node count. Notably, field tests have shown that the single GmDWF1a mutant produced significantly more pods than wild-type plants. Spraying exogenous brassinolide (BL) can compensate for the loss in plant height induced by the decrease in endogenous BRs. Comparing transcriptome analyses of the GmDWF1a mutant and wild-type plants revealed a significant impact on the expression of many genes that influence soybean growth. Identifying the GmDWF1a and GmDWF1b genes could aid in the development of compact, densely planted soybean varieties, potentially boosting productivity.
对 GmDWF1 缺失突变体 dwf1 的研究表明，GmDWF1 通过影响油菜素内酯的生物合成在决定大豆株高和产量方面起着关键作用。大豆没有采用如降低株高以增加种植密度的“绿色革命”，这些方法已被证明对谷类作物有益。我们的研究鉴定了大豆基因 GmDWF1a 和 GmDWF1b，它们与拟南芥 AtDWF1 同源，并发现它们广泛表达，尤其是在叶片中，并与细胞运输系统相关，主要在内质网和细胞内囊泡中。这些基因对于油菜素内酯（BR）的合成是必不可少的。通过 CRISPR/Cas9 基因组编辑产生的 GmDWF1a 和 GmDWF1b 的单突变体以及双突变体均表现出矮化表型。单基因突变体表现出中度矮化，而双突变体则表现出更为明显的矮化。尽管株高降低，但所有类型的突变体都保持其节数不变。值得注意的是，田间试验表明，GmDWF1a 单突变体产生的豆荚明显多于野生型植株。喷洒外源油菜素内酯（BL）可以补偿内源 BR 减少引起的植物高度降低。对 GmDWF1a 突变体和野生型植物的转录组分析进行比较，发现对许多影响大豆生长的基因的表达有显著影响。鉴定 GmDWF1a 和 GmDWF1b 基因可能有助于开发紧凑、密集种植的大豆品种，从而提高生产力。
