College of Forestry, Guizhou University, Guiyang, China.
Biodiversity and Nature Conservation Research Center, Guizhou University, Guiyang, China.
Sci Rep. 2024 Apr 15;14(1):8709. doi: 10.1038/s41598-024-58725-0.
Sect. tuberculata plant belongs to the Camellia genus and is named for the "tuberculiform protuberance on the surface of the ovary and fruit". It is a species of great ornamental value and potential medicinal value. However, little has been reported on the metabolites of C. tuberculata seeds. Therefore, this study was conducted to investigate the metabolites of C. tuberculata seeds based on UPLC/ESI-Q TRAP-MS/MS with extensively targeted metabolomics. A total of 1611 metabolites were identified, including 107 alkaloids, 276 amino acids and derivatives, 283 flavonoids, 86 lignans and coumarins, 181 lipids, 68 nucleotides and derivatives, 101 organic acids, 190 phenolic acids, 10 quinones, 4 steroids, 17 tannins, 111 terpenoids, and 177 other metabolites. We compared the different metabolites in seeds between HKH, ZM, ZY, and LY. The 1311 identified different metabolites were classified into three categories. Sixty-three overlapping significant different metabolites were found, of which lignans and coumarins accounted for the largest proportion. The differentially accumulated metabolites were enriched in different metabolic pathways between HKH vs. LY, HKH vs. ZM, HKH vs. ZY, LY vs. ZY, ZM vs. LY and ZM vs. ZY, with the most abundant metabolic pathways being 4, 2, 4, 7, 7 and 5, respectively (p < 0.05). Moreover, among the top 20 metabolites in each subgroup comparison in terms of difference multiplicity 7, 8 and 13. ZM and ZY had the highest phenolic acid content. Ninety-six disease-resistant metabolites and 48 major traditional Chinese medicine agents were identified based on seven diseases. The results of this study will not only lead to a more comprehensive and in-depth understanding of the metabolic properties of C. tuberculata seeds, but also provide a scientific basis for the excavation and further development of its medicinal value.
节茶叶花属植物因其“子房和果实表面的结核状突起”而得名，是一种具有很高观赏价值和潜在药用价值的物种。然而，关于节茶叶花种子的代谢物报道较少。因此，本研究采用 UPLC/ESI-Q TRAP-MS/MS 结合广泛靶向代谢组学方法对节茶叶花种子的代谢物进行了研究。共鉴定出 1611 种代谢物，包括 107 种生物碱、276 种氨基酸及其衍生物、283 种类黄酮、86 种木脂素和香豆素、181 种脂质、68 种核苷酸及其衍生物、101 种有机酸、190 种酚酸、10 种醌类、4 种甾体、17 种单宁、111 种萜类和 177 种其他代谢物。比较了 HKH、ZM、ZY 和 LY 种子中的不同代谢物。在鉴定的 1311 种不同代谢物中，有 3 种分类。发现 63 个重叠的显著不同代谢物，其中木脂素和香豆素占比最大。差异代谢物在 HKH 与 LY、HKH 与 ZM、HKH 与 ZY、LY 与 ZY、ZM 与 LY 和 ZM 与 ZY 之间的不同代谢途径中富集，最丰富的代谢途径分别为 4、2、4、7、7 和 5（p<0.05）。此外，在每个亚组比较中，差异倍数排名前 20 的代谢物中，7、8 和 13 种的含量最高。ZM 和 ZY 的酚酸含量最高。基于 7 种疾病，共鉴定出 96 种抗病代谢物和 48 种主要中药成分。本研究结果不仅将导致对节茶叶花种子代谢特性的更全面和深入的了解，也为其药用价值的挖掘和进一步开发提供了科学依据。
