Zhang Tao, Ma Xue, Zhou Yuanyuan, Yang Hui, Wang Yuxin, Chen Taolin, Chen Qincao, Deng Yanli
College of Tea, Guizhou University, Jiaxiu South Road, Huaxi District, Guiyang 550025, China.
College of Agriculture, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Qingshan Lake District, Nanchang 330045, China.
Metabolites. 2023 Jun 23;13(7):784. doi: 10.3390/metabo13070784.
The flower is the reproductive organ of the tea plant, while it is also processed into different kinds of products and thus of great significance to be utilized. In this study, the non-volatile secondary metabolites in the internal and external petals of white, white and pink, and pink tea flowers were studied using a widely targeted metabolomics method with ultra-high liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A total of 429 metabolites were identified, including 195 flavonoids, 121 phenolic acids, 40 alkaloids, 29 lignans and coumarins, 19 tannins, 17 terpenoids, and 8 other metabolites. The metabolites in the internal and external petals of different colored flowers showed great changes in flavonoids. Most flavonoids and all tannins in the internal petals were higher compared with the external petals. Some phenolic acids were more accumulated in the external petals, while others showed opposite trends. The pink tea flower contained more flavonoids, alkaloids, lignans, coumarins, terpenoids, and tannins compared with white tea flowers. In addition, cyanidin-3-O-glucoside was more accumulated in the external petals of the pink flower, indicating that anthocyanin may be the main reason for the color difference between the pink and white tea flower. The enriched metabolic pathways of different colored flowers were involved in flavonoid biosynthesis, glycine, serine and threonine metabolism, glycerophospholipid metabolism, and phenylpropanoid biosynthesis. The findings of this study broaden the current understanding of non-volatile compound changes in tea plants. It is also helpful to lay a theoretical foundation for integrated applications of tea flowers.
花是茶树的生殖器官，同时它还可被加工成不同种类的产品，因此具有重要的利用价值。在本研究中，采用超高效液相色谱 - 串联质谱（UPLC-MS/MS）的广泛靶向代谢组学方法，对白茶花、白粉色茶花和粉茶花的内花瓣和外花瓣中的非挥发性次生代谢产物进行了研究。共鉴定出429种代谢产物，包括195种黄酮类化合物、121种酚酸、40种生物碱、29种木脂素和香豆素、19种单宁、17种萜类化合物以及8种其他代谢产物。不同颜色花朵的内花瓣和外花瓣中的代谢产物在黄酮类化合物方面表现出很大变化。与外花瓣相比，内花瓣中的大多数黄酮类化合物和所有单宁含量更高。一些酚酸在外花瓣中积累更多，而另一些则呈现相反的趋势。与白茶花相比，粉茶花含有更多的黄酮类化合物、生物碱、木脂素、香豆素、萜类化合物和单宁。此外，矢车菊素 - 3 - O - 葡萄糖苷在粉花的外花瓣中积累更多，这表明花青素可能是粉茶花和白茶花颜色差异主要原因。不同颜色花朵的富集代谢途径涉及黄酮类生物合成、甘氨酸、丝氨酸和苏氨酸代谢、甘油磷脂代谢以及苯丙烷生物合成。本研究结果拓宽了目前对茶树中非挥发性化合物变化的认识。这也有助于为茶花的综合应用奠定理论基础。
