Ryan J L, Yohe H C, Malech H L
Yale J Biol Med. 1985 Mar-Apr;58(2):125-31.
Membrane ganglioside changes in murine peritoneal macrophages and the human promyelocytic leukemia cell line HL-60 have been assessed by two-dimensional thin-layer chromatography. C3H/HeJ mice respond to protein-containing endotoxin but are hyporesponsive to protein-free endotoxin preparations. Compared to unstimulated resident cells, protein-containing endotoxin produced an alteration in the C3H/HeJ macrophage ganglioside pattern whereas protein-free endotoxin did not. In comparison, differentiation of HL-60 cells to a neutrophil-like cell by dimethylsulfoxide gave a ganglioside pattern similar to unstimulated HL-60 cells. However, differentiation of HL-60 cells by phorbol myristate acetate to macrophage-like cells results in a large increase in the monosialoganglioside GM3. The evidence presented indicates that discrete ganglioside changes occur in murine monocytes and HL-60 cells upon induction to cells with increased macrophage functions.
已通过二维薄层色谱法评估了小鼠腹膜巨噬细胞和人早幼粒细胞白血病细胞系HL-60中的膜神经节苷脂变化。C3H/HeJ小鼠对含蛋白质的内毒素有反应，但对无蛋白质的内毒素制剂反应低下。与未刺激的驻留细胞相比，含蛋白质的内毒素使C3H/HeJ巨噬细胞神经节苷脂模式发生改变，而无蛋白质的内毒素则没有。相比之下，二甲基亚砜使HL-60细胞分化为嗜中性粒细胞样细胞，其神经节苷脂模式与未刺激的HL-60细胞相似。然而，佛波醇肉豆蔻酸酯乙酸盐使HL-60细胞分化为巨噬细胞样细胞会导致单唾液酸神经节苷脂GM3大幅增加。所提供的证据表明，在诱导小鼠单核细胞和HL-60细胞转变为具有增强巨噬细胞功能的细胞时，会发生离散的神经节苷脂变化。
