Saito M, Terui Y, Nojiri H
Biochem Biophys Res Commun. 1985 Oct 15;132(1):223-31. doi: 10.1016/0006-291x(85)91011-3.
A remarkable increase in monosialo-ganglioside GM3 was observed during the monocytic differentiation of HL-60 cells induced by 12-0-tetradecanoyl-phorbol-13-acetate(TPA). On the other hand, when the cells were cultured with exogenously-added ganglioside GM3 in serum-free conditions, their differentiation along a monocytic lineage was demonstrated with simultaneous complete growth inhibition. Other gangliosides such as ganglioside GM1 showed no effects on cell differentiation, exhibiting instead stimulatory actions on the cell growth. These results indicate that a physiologically-existent, membranous ganglioside GM3, which specifically increases during monocytic cell differentiation, might play a primary role as a trigger in the monocytic cell differentiation.
在用12-0-十四烷酰佛波醇-13-乙酸酯（TPA）诱导HL-60细胞单核细胞分化的过程中，观察到单唾液酸神经节苷脂GM3显著增加。另一方面，当细胞在无血清条件下与外源添加的神经节苷脂GM3一起培养时，它们沿单核细胞谱系的分化得到证实，同时细胞生长完全受到抑制。其他神经节苷脂，如神经节苷脂GM1，对细胞分化没有影响，反而对细胞生长有刺激作用。这些结果表明，在单核细胞分化过程中特异性增加的生理性存在的膜性神经节苷脂GM3可能作为单核细胞分化的触发因素发挥主要作用。
