Department of Nuclear Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, P.R. China.
Department of Nuclear Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, P.R. China.
PLoS One. 2024 Apr 16;19(4):e0298631. doi: 10.1371/journal.pone.0298631. eCollection 2024.
Endothelial specific molecule-1 (ESM1) is implicated as an oncogene in multiple human cancers. However, the function of ESM1 in papillary thyroid cancer (PTC) is not well understood. The current study aimed to investigate the effect of ESM1 on the growth, migration, and invasion of PTC to provide a novel perspective for PTC treatment.
The expression levels of ESM1 in PTC tissues form 53 tumor tissue samples and 59 matching adjacent normal tissue samples were detected by immunohistochemical analysis. Knockdown of ESM1 expression in TPC-1 and SW579 cell lines was established to investigate its role in PTC. Moreover, cell proliferation, apoptosis, wound healing, and transwell assays were conducted in vitro to assess cell proliferation, migration and invasion.
The findings revealed that ESM1 expression was significantly higher in PTC tissues than that found in paraneoplastic tissues (P<0.0001). Knockdown of ESM1 expression inhibited the proliferation, migration, and invasion of TPC-1 and SW579 cells in vitro. Compared with the control group, the mRNA and protein levels of ESM1 in PTC cells were significantly reduced following knockdown of its expression (P<0.01). In addition, ESM1-knockdown cells indicated decreased proliferation and decreased migratory and invasive activities (P<0.01, P<0.01, P<0.001, respectively).
ESM1 was identified as a major gene in the occurrence and progression of PTC, which could increase the proliferation, migration, and invasion of PTC cells. It may be a promising diagnostic and therapeutic target gene.
内皮细胞特异性分子-1（ESM1）被认为是多种人类癌症的癌基因。然而，ESM1 在甲状腺乳头状癌（PTC）中的功能尚不清楚。本研究旨在探讨 ESM1 对 PTC 生长、迁移和侵袭的影响，为 PTC 治疗提供新视角。
通过免疫组织化学分析检测 53 例肿瘤组织样本和 59 例配对的相邻正常组织样本中 ESM1 的表达水平。建立 TPC-1 和 SW579 细胞系中 ESM1 表达的敲低模型，以研究其在 PTC 中的作用。此外，进行体外细胞增殖、凋亡、划痕愈合和 Transwell 测定，以评估细胞增殖、迁移和侵袭。
研究结果表明，ESM1 在 PTC 组织中的表达明显高于癌旁组织（P<0.0001）。ESM1 表达的敲低抑制了 TPC-1 和 SW579 细胞的体外增殖、迁移和侵袭。与对照组相比，ESM1 表达敲低后 PTC 细胞中的 ESM1 mRNA 和蛋白水平均显著降低（P<0.01）。此外，ESM1 敲低细胞显示增殖减少，迁移和侵袭活性降低（P<0.01、P<0.01、P<0.001）。
ESM1 被鉴定为 PTC 发生和进展的主要基因，可增加 PTC 细胞的增殖、迁移和侵袭。它可能是一个有前途的诊断和治疗靶基因。
