Loegering D J, Moon D G, Kaplan J E, Weber P, Blumenstock F A
J Leukoc Biol. 1985 Nov;38(5):613-24. doi: 10.1002/jlb.38.5.613.
This study was carried out to evaluate the mechanism of action of a reticuloendothelial (RE)-depressing substance. This RE-depressing substance was obtained from the plasma of dogs subjected to 3 hr of intestinal ischemia. RE-depressing substance was partially purified by dialysis and reverse-phase column chromatography. The assay of RE-depressing activity was based on the depression of the rate of clearance of colloidal carbon from the blood of rats or mice. The effect of RE-depressing substance on three other RE system (RES) test particles (gelatinized lipid emulsion, formalinized sheep erythrocytes, and IgM-coated erythrocytes) was determined. RE-depressing substance did not affect the clearance rate or the organ localization of these three test particles. Therefore, RE-depressing substance affected only the clearance of colloidal carbon. Since platelet aggregation has been shown to contribute to the clearance of colloidal carbon, the effect of RE-depressing substance on platelet aggregation was evaluated. RE-depressing substance depressed in vitro platelet aggregation induced by ADP or collagen. It was concluded that the effect of RE-depressing substance on the clearance of colloidal carbon was due to a depression of platelet aggregation rather than to a depression of hepatic macrophage phagocytic function.
本研究旨在评估一种网状内皮系统（RE）抑制物质的作用机制。这种RE抑制物质是从经历3小时肠缺血的犬血浆中获得的。通过透析和反相柱色谱法对RE抑制物质进行了部分纯化。RE抑制活性的测定基于大鼠或小鼠血液中胶体碳清除率的降低。测定了RE抑制物质对其他三种网状内皮系统（RES）测试颗粒（糊化脂质乳剂、甲醛化绵羊红细胞和IgM包被红细胞）的影响。RE抑制物质不影响这三种测试颗粒的清除率或器官定位。因此，RE抑制物质仅影响胶体碳的清除。由于血小板聚集已被证明有助于胶体碳的清除，因此评估了RE抑制物质对血小板聚集的影响。RE抑制物质可抑制ADP或胶原诱导的体外血小板聚集。得出的结论是，RE抑制物质对胶体碳清除的影响是由于血小板聚集的抑制，而不是肝巨噬细胞吞噬功能的抑制。
