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与牛羊水间充质干细胞多能性相关的免疫调节基因转录本。

Immunomodulatory-associated gene transcripts to multipotency of bovine amniotic fluid mesenchymal stem cells.

作者信息

Baptistella Jamila Cristina, da Silva Carolina Gonzales, Báo Sônia Nair, Panegossi Letícia Colin, Cardoso Tereza Cristina, de Carvalho Roberto Gameiro, Martins Carlos Frederico

机构信息

Laboratório de Virologia e Cultura Celular, Faculdade de Medicina Veterinária, Universidade Estadual Paulista - UNESP, Araçatuba, SP, Brasil.

Faculdade de Medicina Veterinária, Centro Universitário Católico Salesiano - UniSalesiano, Araçatuba, SP, Brasil.

出版信息

Anim Reprod. 2024 Apr 12;21(1):e20230155. doi: 10.1590/1984-3143-AR2023-0155. eCollection 2024.

DOI:10.1590/1984-3143-AR2023-0155
PMID:38628495
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11019794/
Abstract

The adnexa fetal tissues are sources of mesenchymal stromal cells (MSCs) due to their noninvasive harvest, with all biological material discarded most of the time. MSCs are a promise regarding to their plasticity, self-renewal, differentiation potentials, immunomodulatory and anti-inflammatory properties, which have made clinical stem cell therapy a reality. The present study aimed to characterize and evaluate the immunomodulation ability of bovine mesenchymal cells collected from bovine amniotic fluid (bAFMSCs) isolated and subjected to sixth consecutive culture passages . The multilineage properties of the bAFMSCs collections confirmed the ability to undergo adipogenic, chondrogenic and osteogenic differentiation. The mesenchymal gene transcription were detected in bAFMSCs, whereas and were not detected. Regarding cytokine mRNA expression, and were downregulated, while was highly regulated in all studied passages. The present study demonstrated the immunological properties and multipotency of bAFMSCs collections, and thus, they can be tested in cattle pathological treatments or multiplication by nuclear transfer cloning.

摘要

胎儿附属组织是间充质基质细胞（MSCs）的来源，因为它们可以无创获取，且大部分生物材料在大多数情况下都会被丢弃。MSCs因其可塑性、自我更新能力、分化潜能、免疫调节和抗炎特性而颇具前景，这些特性已使临床干细胞治疗成为现实。本研究旨在对从牛羊水分离并连续传代培养至第六代的牛间充质细胞（bAFMSCs）的免疫调节能力进行表征和评估。bAFMSCs群体的多谱系特性证实了其具有向脂肪细胞、软骨细胞和成骨细胞分化的能力。在bAFMSCs中检测到了间充质基因转录，而未检测到[此处原文缺失具体基因名称]和[此处原文缺失具体基因名称]。关于细胞因子mRNA表达，[此处原文缺失具体基因名称]和[此处原文缺失具体基因名称]表达下调，而[此处原文缺失具体基因名称]在所有研究传代中均高度表达。本研究证明了bAFMSCs群体的免疫特性和多能性，因此，它们可用于牛的病理治疗测试或通过核移植克隆进行增殖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ca/11019794/603c6353476e/1984-3143-ar-21-1-e20230155-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ca/11019794/cdfaee3a049f/1984-3143-ar-21-1-e20230155-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ca/11019794/6fc2f9823e59/1984-3143-ar-21-1-e20230155-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ca/11019794/603c6353476e/1984-3143-ar-21-1-e20230155-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ca/11019794/cdfaee3a049f/1984-3143-ar-21-1-e20230155-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ca/11019794/6fc2f9823e59/1984-3143-ar-21-1-e20230155-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66ca/11019794/603c6353476e/1984-3143-ar-21-1-e20230155-gf03.jpg

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Mol Biol Rep. 2021 May;48(5):3913-3923. doi: 10.1007/s11033-021-06383-0. Epub 2021 May 28.
2
Immunomodulatory and immunogenic properties of mesenchymal stem cells derived from bovine fetal bone marrow and adipose tissue.牛胎儿骨髓和脂肪组织来源间充质干细胞的免疫调节和免疫原性。
Res Vet Sci. 2019 Jun;124:212-222. doi: 10.1016/j.rvsc.2019.03.017. Epub 2019 Mar 21.
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Stem cells on regenerative and reproductive science in domestic animals.
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Vet Res Commun. 2019 Feb;43(1):7-16. doi: 10.1007/s11259-019-9744-6. Epub 2019 Jan 17.
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Production of Bovine Embryos and Calves Cloned by Nuclear Transfer Using Mesenchymal Stem Cells from Amniotic Fluid and Adipose Tissue.利用羊水和脂肪组织来源的间充质干细胞通过核移植克隆牛胚胎和犊牛的生产。
Cell Reprogram. 2016 Apr;18(2):127-36. doi: 10.1089/cell.2015.0064.
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Canine placenta: A promising potential source of highly proliferative and immunomodulatory mesenchymal stromal cells?犬胎盘：高度增殖和免疫调节间充质基质细胞的一个有前景的潜在来源？
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