Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P, India.
Division of Veterinary Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P, India.
Mol Biol Rep. 2021 May;48(5):3913-3923. doi: 10.1007/s11033-021-06383-0. Epub 2021 May 28.
The caprine mesenchymal stem cells (MSCs) derived from fetal adnexa are highly proliferative. These cells possess tri-lineage differentiation potential and express MSC surface antigens and pluripotency markers with a wound-healing potential. This present study was conducted to compare the immunomodulatory potential of caprine MSCs derived from the fetal adnexa. Mid-gestation caprine uteri (2-3 months) were collected from the abattoir to isolate MSCs from amniotic fluid (cAF), amniotic sac (cAS), Wharton's jelly (cWJ) and cord blood (cCB), which were expanded and characterized at the 3 passage. These MSCs were then stimulated with inflammatory cytokines (IFN-γ and TNF-α) to assess the percentage of inhibition produced on peripheral blood mononuclear cells (PBMCs) proliferation. The percentage of inhibition on activated PBMCs proliferation produced by cWJ MSCs and cAS MSCs was significantly higher than cCB and cAF MSCs. The relative mRNA expression profile and immunofluorescent localization of different immunomodulatory cytokines and growth factors were conducted upon stimulation. The mRNA expression profile of a set of different cytokines and growth factors in each caprine fetal adnexa MSCs were modulated. Indoleamine 2, 3 dioxygenase appeared to be the major immunomodulator in cWJ, cAF, and cCB MSCs whereas inducible nitric oxide synthase in cAS MSCs. This study suggests that caprine MSCs derived from fetal adnexa display variable immunomodulatory potential, which appears to be modulated by different molecules among sources.
山羊胎儿附属物来源的间充质干细胞（MSCs）具有高度增殖能力。这些细胞具有三系分化潜能，并表达 MSC 表面抗原和多能性标志物，具有伤口愈合潜能。本研究旨在比较来源于胎儿附属物的山羊 MSCs 的免疫调节潜能。从中期妊娠山羊子宫（2-3 个月）中收集羊水（cAF）、羊膜囊（cAS）、华通氏胶（cWJ）和脐血（cCB），从这些组织中分离 MSCs 并进行扩增和表型鉴定，在第 3 代时进行。然后用炎性细胞因子（IFN-γ 和 TNF-α）刺激这些 MSC，以评估其对外周血单个核细胞（PBMCs）增殖的抑制百分比。cWJ MSC 和 cAS MSC 对活化的 PBMCs 增殖的抑制百分比明显高于 cCB 和 cAF MSC。在刺激后进行不同免疫调节细胞因子和生长因子的相对 mRNA 表达谱和免疫荧光定位。对每一种来源于山羊胎儿附属物的 MSC 中的一组不同的细胞因子和生长因子的 mRNA 表达谱进行了调节。吲哚胺 2,3 双加氧酶似乎是 cWJ、cAF 和 cCB MSC 中的主要免疫调节剂，而诱导型一氧化氮合酶则是 cAS MSC 中的主要免疫调节剂。本研究表明，来源于胎儿附属物的山羊 MSCs 显示出不同的免疫调节潜能，这种潜能似乎是由不同来源的分子调节的。
