Yamashina K, Fulton A, Heppner G
J Natl Cancer Inst. 1985 Oct;75(4):765-70.
Sensitivity to macrophage-mediated cytostasis was determined with 4 tumor cell lines derived from a single, spontaneously arising mouse mammary tumor. Cytostasis was measured in a 48-hour [3H]thymidine-incorporation assay with the use of maleic vinyl ether (pyran) fraction 2 (MVE-2)-elicited peritoneal macrophages as effector cells. Metastatic tumor lines 66 and 410.4 were less sensitive than nonmetastatic lines 67 and 168. Pretreatment of tumor cells with indomethacin for 24 hours before assay increased the cytostatic sensitivity of the metastatic tumor lines but did not affect that of the nonmetastatic tumor lines. Addition of 100 ng lipopolysaccharide (LPS)/ml to the assay mixture of MVE-2-primed macrophages and tumor cells or pretreatment of macrophages with LPS markedly lessened the differences in cytostatic sensitivity among the metastatic and nonmetastatic lines. Pretreatment of tumor cells with indomethacin plus addition of LPS during the effector phase of the assay completely abrogated differences in sensitivity. These results suggest that differences in sensitivity of metastatic versus nonmetastatic tumor cells to macrophage cytostasis are due to both tumor cell (prostaglandin) and effector cell (activation state) factors.
利用源自一只自发产生的小鼠乳腺肿瘤的4种肿瘤细胞系，测定了对巨噬细胞介导的细胞生长抑制作用的敏感性。在一项48小时的[3H]胸苷掺入试验中，以马来酸乙烯醚（吡喃）组分2（MVE-2）诱导的腹腔巨噬细胞作为效应细胞来测定细胞生长抑制作用。转移性肿瘤细胞系66和410.4比非转移性细胞系67和168敏感性更低。在试验前用吲哚美辛对肿瘤细胞预处理24小时，可提高转移性肿瘤细胞系的细胞生长抑制敏感性，但不影响非转移性肿瘤细胞系的敏感性。向MVE-2预处理的巨噬细胞与肿瘤细胞的试验混合物中添加100 ng脂多糖（LPS）/ml，或用LPS对巨噬细胞进行预处理，可显著减小转移性和非转移性细胞系之间细胞生长抑制敏感性的差异。在试验的效应阶段，用吲哚美辛对肿瘤细胞进行预处理并添加LPS，可完全消除敏感性差异。这些结果表明，转移性与非转移性肿瘤细胞对巨噬细胞介导的细胞生长抑制作用的敏感性差异，是由肿瘤细胞（前列腺素）和效应细胞（激活状态）因素共同导致的。
