Ajibare Ayodeji Johnson, Akintoye Olabode Oluwadare, Folawiyo Moshood Abiola, Babalola Kabirat Temitope, Omotuyi Olaposi Idowu, Oladun Busayo Timothy, Aransi-Ola Kafilat Temidayo, Odetayo Adeyemi Fatai, Olayaki Luqman Aribidesi
Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine and Health Sciences, Lead City University Ibadan, Oyo State, Nigeria.
Department of Physiology, Ekiti-State University, Ado-Ekiti, Ekiti-State, Nigeria.
Iran J Basic Med Sci. 2024;27(5):543-551. doi: 10.22038/IJBMS.2024.71288.15484.
Male infertility is a major public health issue due to increased prevalence, so there is an urgent need for a therapeutic solution. The search for a natural dietary substance that could modulate redox balance and inflammation and protect testicular function is in demand. Virgin Coconut Oil (VCO) has found use in the treatment of diabetes, and cancer owing to the presence of polyphenols. However, there is a dearth of information on its effect on testicular toxicity. The present study investigated VCO as a possible treatment for testicular toxicity in the Sodium Benzoate (SB) model of male infertility by evaluating the oxidative and inflammatory status, circulating hormonal levels, and key sperm indices.
Twenty adult male rats were randomly assigned to four groups of 5 rats each and were treated with normal saline, sodium benzoate, sodium benzoate+5% VCO, and sodium benzoate+15% VCO for 30 days respectively. Biochemical analysis of reproductive hormones was assessed. Sperm parameters assessed include sperm function tests and sperm kinematics. One-way analysis of variance (ANOVA) followed by post hoc Tukey tests was performed.
5% VCO reverts the deranged serum reproductive hormones caused by sodium benzoate. 5% VCO was more potent as an antioxidant and anti-inflammatory treatment than 15% VCO. However, both doses prevented SB's effect on the sperm function test and kinematics.
VCO-supplemented diet can ameliorate SB-induced testicular toxicity by inhibiting its mechanisms of toxicity that are related to oxidative stress, apoptosis, and inflammation.
由于男性不育症的患病率上升，它已成为一个重大的公共卫生问题，因此迫切需要一种治疗方案。人们急需找到一种天然的饮食物质，它能够调节氧化还原平衡和炎症反应，并保护睾丸功能。初榨椰子油（VCO）因含有多酚类物质，已被用于治疗糖尿病和癌症。然而，关于其对睾丸毒性影响的信息却很匮乏。本研究通过评估氧化和炎症状态、循环激素水平以及关键精子指标，研究了VCO作为一种可能治疗苯甲酸钠（SB）所致男性不育模型中睾丸毒性的方法。
将20只成年雄性大鼠随机分为四组，每组5只，分别用生理盐水、苯甲酸钠、苯甲酸钠+5% VCO和苯甲酸钠+15% VCO处理30天。对生殖激素进行生化分析。评估的精子参数包括精子功能测试和精子运动学。进行单因素方差分析（ANOVA），随后进行事后Tukey检验。
5% VCO可逆转苯甲酸钠引起的血清生殖激素紊乱。作为抗氧化和抗炎治疗，5% VCO比15% VCO更有效。然而，两种剂量均能防止SB对精子功能测试和运动学的影响。
补充VCO的饮食可通过抑制与氧化应激、细胞凋亡和炎症相关的毒性机制，改善SB诱导的睾丸毒性。
