sequence type 147: a high-risk clone increasingly associated with plasmids carrying both resistance and virulence elements.

The first hybrid resistance/virulence plasmid, combining elements from virulence plasmids described in hypervirulent types of with those from conjugative resistance plasmids, was described in an isolate of sequence type (ST) 147 from 2016. Subsequently, this type has been increasingly associated with these plasmids. The extent of carriage of hybrid virulence/resistance plasmids in nosocomial isolates of requires further investigation. To describe the occurrence of virulence/resistance plasmids among isolates of received by the UK reference laboratory, particularly among representatives of ST147, and to compare their sequences. Isolates received by the laboratory during 2022 and the first half of 2023 (=1278) were screened for virulence plasmids by PCR detection of / and typed by variable-number tandem repeat analysis. Twenty-nine representatives of ST147 (including a single-locus variant) from seven hospital laboratories were subjected to long-read nanopore sequencing using high-accuracy q20 chemistry to provide complete assemblies. / were detected in 110 isolates, of which 59 belonged to hypervirulent K1-ST23, K2-ST86 and K2-ST65/375. Of the remainder, representatives of ST147 formed the largest group, with 22 /-positive representatives (out of 47 isolates). Representatives were from 19 hospital laboratories, with /-positive isolates from 10. Nanopore sequencing of 29 representatives of ST147 divided them into those with no virulence plasmid (=12), those with non-New Delhi metallo-β-lactamase (NDM) virulence plasmids (=6) and those carrying (=9) or (=2) virulence plasmids. These plasmids were of IncFIB(pNDM-Mar)/IncHI1B(pNDM-MAR) replicon types. Most of the non-NDM virulence plasmids were highly similar to the originally described KpvST147L_NDM plasmid. Those carrying were highly similar to one another and to previously described plasmids in ST383 and carried an extensive array of resistance genes. Comparison of the fully assembled chromosomes indicated multiple introductions of ST147 in UK hospitals. This study highlights the high proportion of representatives of ST147 that carry IncFIB(pNDM-Mar)/IncHI1B(pNDM-MAR) hybrid resistance virulence plasmids. It is important to be aware of the high probability that representatives of this type carry these plasmids combining resistance and virulence determinants and of the consequent increased risk to patients.