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由具有序列型 29 的高毒力 携带。

carried by a hypervirulent with sequence type 29.

机构信息

Department of Laboratory Medicine, The First People's Hospital of Neijiang, Neijiang, Sichuan China.

2Department of Immunology, School of Basic Medical Sciences, Southwest Medical University, Luzhou, Sichuan China.

出版信息

Antimicrob Resist Infect Control. 2019 Aug 19;8:140. doi: 10.1186/s13756-019-0596-1. eCollection 2019.

DOI:10.1186/s13756-019-0596-1
PMID:31452874
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6701021/
Abstract

BACKGROUND

A carbapenem-resistant hypermucoviscous isolate was recovered from human sputum.

METHODS

Whole genome sequencing of this isolate was carried out to reveal its clonal background, antimicrobial resistance determinants and virulence factors. Virulence assays were performed using wax moth larvae. The transfer of between bacterial strains was tested using conjugation. 59 genome assemblies of ST29 and 230 IncX3 plasmids regardless of the carriage of resistance gene were employed for phylogenetic analysis, respectively.

RESULTS

The strain carried a virulence plasmid pVir-SCNJ1 bearing the virulence gene and exhibited a high virulence in wax moth. This hypervirulent strain belongs to sequence type 29 and carries , which is located on a conjugative plasmid, designated pNDM5-SCNJ1, belonging to type IncX3. pNDM5-SCNJ1 was fully sequenced and shows high similarity with pNDM_MGR194, except some deletion inside the IS region. Phylogenetic analysis of IncX3 plasmids revealed that although can be evolved from via point mutations within some IncX3 plasmids, most of -carrying IncX3 plasmids probably have acquired in multiple events.

CONCLUSIONS

In this study, we characterized a -positive hypervirulent of sequence type 29 in China. Our results highlight the need for active surveillance on this lineage of carbapenem-resistant .

摘要

背景

从人类痰液中分离出一株耐碳青霉烯的高粘液性 。

方法

对该分离株进行全基因组测序，以揭示其克隆背景、抗微生物药物耐药决定因子和毒力因子。使用黄粉虫幼虫进行毒力试验。使用接合试验检测 之间的转移。分别使用 59 个 ST29 基因组组装和 230 个 IncX3 质粒（无论是否携带耐药基因）进行系统发育分析。

结果

该菌株携带一个携带毒力基因 的毒力质粒 pVir-SCNJ1，并在黄粉虫中表现出高毒力。这种高毒力菌株属于序列类型 29，携带 ，它位于一个可接合的质粒上，命名为 pNDM5-SCNJ1，属于 IncX3 型。pNDM5-SCNJ1 已被完全测序，与 pNDM_MGR194 高度相似，除了 IS 区内部的一些缺失。IncX3 质粒的系统发育分析表明，尽管 可以通过某些 IncX3 质粒中的点突变从 进化而来，但大多数携带 的 IncX3 质粒可能是通过多次事件获得的。

结论

本研究在中国首次描述了一株耐碳青霉烯的高粘液性 序列类型 29 的 阳性菌株。我们的结果强调了需要对这种碳青霉烯耐药 进行积极监测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a32/6701021/18ac13aa04e7/13756_2019_596_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a32/6701021/2991b4c624f8/13756_2019_596_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a32/6701021/afe4767300df/13756_2019_596_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a32/6701021/05256282b0e5/13756_2019_596_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a32/6701021/c5b1da4ac672/13756_2019_596_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a32/6701021/18ac13aa04e7/13756_2019_596_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a32/6701021/2991b4c624f8/13756_2019_596_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a32/6701021/afe4767300df/13756_2019_596_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a32/6701021/05256282b0e5/13756_2019_596_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a32/6701021/c5b1da4ac672/13756_2019_596_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a32/6701021/18ac13aa04e7/13756_2019_596_Fig5_HTML.jpg

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2
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Front Microbiol. 2018 Oct 25;9:2568. doi: 10.3389/fmicb.2018.02568. eCollection 2018.
3
Occurrence of colistin-resistant hypervirulent Klebsiella variicola.高产黏菌素耐药性弗氏柠檬酸杆菌的出现。
荚膜突变是K54高毒力肺炎克雷伯菌噬菌体抗性进化的关键策略。
Commun Biol. 2025 Feb 18;8(1):257. doi: 10.1038/s42003-025-07687-8.
4
Hypervirulent (hv): Overview, Epidemiology, and Laboratory Detection.高毒力（hv）：概述、流行病学及实验室检测
Pathog Immun. 2025 Jan 24;10(1):80-119. doi: 10.20411/pai.v10i1.777. eCollection 2024.
5
Molecular Characteristics of Virulence Genes in Carbapenem-Resistant and Carbapenem-Sensitive in Relation to Different Capsule Serotypes in Ningbo, China.中国宁波耐碳青霉烯类与碳青霉烯类敏感菌株中与不同荚膜血清型相关的毒力基因分子特征
Infect Drug Resist. 2024 May 27;17:2109-2120. doi: 10.2147/IDR.S461666. eCollection 2024.
6
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Front Microbiol. 2024 Feb 7;15:1356229. doi: 10.3389/fmicb.2024.1356229. eCollection 2024.
7
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4
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Pathog Dis. 2017 Dec 29;75(9). doi: 10.1093/femspd/ftx121.
7
A fatal outbreak of ST11 carbapenem-resistant hypervirulent Klebsiella pneumoniae in a Chinese hospital: a molecular epidemiological study.中国医院中致命的 ST11 碳青霉烯类耐药超毒力肺炎克雷伯菌爆发：一项分子流行病学研究。
Lancet Infect Dis. 2018 Jan;18(1):37-46. doi: 10.1016/S1473-3099(17)30489-9. Epub 2017 Aug 29.
8
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Lancet Infect Dis. 2018 Jan;18(1):2-3. doi: 10.1016/S1473-3099(17)30517-0. Epub 2017 Aug 29.
9
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