Universidade do Estado do Rio de Janeiro - Biomedical Center - Department of Anatomy - Rio de Janeiro (RJ) - Brazil.
Universidade do Estado do Rio de Janeiro - Biomedical Center - Department of Microbiology, Immunology and Parasitology - Rio de Janeiro (RJ) - Brazil.
Acta Cir Bras. 2024 Apr 15;39:e392224. doi: 10.1590/acb392224. eCollection 2024.
To evaluate, by quantitative and qualitative methods, the glomerular ultrastructure in Wistar rats fed a cafeteria diet.
Male Wistar rats were divided into two groups at 21 days of age: control (C, n = 10) and cafeteria diet (CAF, n = 8). The animals were followed up until 5 months of age, followed by euthanasia. The blood, kidneys, and fat deposits--epididymal, retroperitoneal, and subcutaneous--were extracted and analyzed. Data were analyzed by Student's t test, and p < 0.05 was considered statistically significant.
The cafeteria diet promoted glucose intolerance, hyperglycemia (p < 0.0001), and deposition of retroperitoneal fat (p < 0.005). Scanning electron microscopy revealed that the length of the foot process was similar in both groups. The quantitative analyses by transmission electron microscopy revealed that the cafeteria diet reduced the thickness of the glomerular basement membrane (p < 0.05).
The intake of lipids and simple carbohydrates were found to be associated with alteration in the glomerular ultrastructure. However, more studies are needed to evaluate not only the effects of high-protein and high-fat diets on components of the glomerular filtration barrier, but also renal physiology.
通过定量和定性方法评估给予 cafeteria 饮食的 Wistar 大鼠的肾小球超微结构。
21 日龄雄性 Wistar 大鼠分为两组：对照组（C，n = 10）和 cafeteria 饮食组（CAF，n = 8）。动物被跟踪至 5 月龄，随后处死。提取血液、肾脏和脂肪组织（附睾、腹膜后和皮下）并进行分析。数据采用 Student's t 检验进行分析，p < 0.05 被认为具有统计学意义。
cafeteria 饮食导致葡萄糖不耐受、高血糖（p < 0.0001）和腹膜后脂肪沉积（p < 0.005）。扫描电子显微镜显示两组大鼠足突长度相似。透射电子显微镜的定量分析显示 cafeteria 饮食降低了肾小球基底膜的厚度（p < 0.05）。
摄入脂质和简单碳水化合物与肾小球超微结构改变有关。然而，需要更多的研究来评估高蛋白和高脂肪饮食对肾小球滤过屏障成分的影响，以及肾脏生理学。
