Kansai Gakken Laboratory, Kankyo Eisei Yakuhin Co. Led, Seika-cho, Kyoto, Japan.
Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan.
Methods Mol Biol. 2024;2794:305-311. doi: 10.1007/978-1-0716-3810-1_25.
Brain defects often lead to motor dysfunctions in humans. Drosophila melanogaster has been one of the most useful organisms in the study of neuronal biology due to its similarities with humans and has contributed to a more detailed understanding of the effects of genetic dysfunctions in the brain on behavior. We herein present modified protocols for the crawling assay with larvae and the climbing assay with adult flies that are simple to perform as well as a series of commands for ImageJ to automatically analyze data for the crawling assay.
脑缺陷常导致人类运动功能障碍。果蝇由于与人类的相似性，已成为神经生物学研究中最有用的生物之一，并有助于更详细地了解大脑中遗传功能障碍对行为的影响。本文提出了改良的幼虫爬行试验和成年蝇攀爬试验方案，这些方案简单易行，并提供了一系列 ImageJ 命令，用于自动分析爬行试验的数据。
