School of Biological Sciences and Institute of Microbiology, Seoul National University, Building 504-421, Seoul, 08826, South Korea.
Center for RNA Research, Institute for Basic Science, Seoul, 08826, South Korea.
J Membr Biol. 2024 Aug;257(3-4):207-214. doi: 10.1007/s00232-024-00312-5. Epub 2024 Apr 17.
The signal peptidase complex (SPC) mediates processing of signal peptides of secretory precursors. But, recent studies show that the eukaryotic SPC also cleaves internal transmembrane segments of some membrane proteins, and its non-catalytic subunit, Spc1/SPCS1 plays a critical role in this process. To assess the impact of Spc1 on membrane proteostasis, we carried out quantitative proteomics of yeast cells with and without Spc1. Our data show that the abundance of the membrane proteome in yeast cells lacking Spc1 is in general reduced compared to that in wild-type cells, implicating its role in controlling the cellular levels of membrane proteins.
信号肽酶复合物（SPC）介导分泌前体的信号肽的加工。但是，最近的研究表明，真核 SPC 也切割一些膜蛋白的内部跨膜片段，其非催化亚基 Spc1/SPCS1 在这个过程中起着关键作用。为了评估 Spc1 对膜蛋白稳态的影响，我们对有和没有 Spc1 的酵母细胞进行了定量蛋白质组学研究。我们的数据表明，与野生型细胞相比，缺乏 Spc1 的酵母细胞中膜蛋白组的丰度通常降低，这表明它在控制膜蛋白的细胞水平方面发挥作用。
