Farouk Hala Montaser, Attia Eman Zekry, Shaban Gehan Mohamed, Abdelmohsen Usama Ramadan, El-Katatny Mo'men Hamed
Botany and Microbiology Department, Faculty of Science, Minia University, Minia, Egypt.
Pharmacognosy Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Nat Prod Res. 2024 Apr 17:1-5. doi: 10.1080/14786419.2024.2340761.
A total of 20 endophytic fungi were isolated (ZSEFL1-ZSEFL20) from (L.) Desf. (Nabq) leaves. Four isolates A2/ZSEFL2, , D/ZSEFL14, , E/ZSEFL15, , and S/ZSEFL19, were found to show the most promising antimicrobial activities either in plug or disc diffusion screening assays against Gram-positive, Gram-negative bacteria and pathogenic fungi. Antimicrobial activity was tested against ATCC 27853, ATCC 29213, ATCC 14764, e ATCC 700603, ATCC 10231, and ATCC417. antioxidant activity assay was conducted using the ABTS [2,2'-Azino-bis (3-Ethylbenzthiazoline-6-Sulfonic Acid)] free radical scavenging method. EtOAc extracts of all isolated endophytic fungi showed antioxidant activities. This study would be one of the first reports to measure the antioxidant activity of (L.) Desf. endophytic fungi. Therefore, these isolated endophytic fungi can provide additional information for medicinal sources of natural antioxidants and antimicrobial agents.
从（某种植物学名，未完整给出）（纳布克）叶片中共分离出20种内生真菌（ZSEFL1 - ZSEFL20）。在琼脂块或纸片扩散筛选试验中，发现4种分离物A2/ZSEFL2、D/ZSEFL14、E/ZSEFL15和S/ZSEFL19对革兰氏阳性菌、革兰氏阴性菌和致病真菌表现出最具前景的抗菌活性。针对金黄色葡萄球菌ATCC 27853、大肠埃希菌ATCC 29213、白色念珠菌ATCC 14764、粪肠球菌ATCC 700603、枯草芽孢杆菌ATCC 10231和黑曲霉ATCC417测试了抗菌活性。使用ABTS [2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)]自由基清除法进行抗氧化活性测定。所有分离出的内生真菌的乙酸乙酯提取物均显示出抗氧化活性。本研究将是首批测定（该植物学名，未完整给出）内生真菌抗氧化活性的报告之一。因此，这些分离出的内生真菌可为天然抗氧化剂和抗菌剂的药用来源提供更多信息。
