Jalouzot R, Toublan B, Wilhelm M L, Wilhelm F X
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6475-9. doi: 10.1073/pnas.82.19.6475.
The time of replication of the two H4 histone genes (H41 and H42) was determined during the naturally synchronous mitotic cycle of Physarum polycephalum. 5-Bromo-2'-deoxyuridine labeling and density gradient centrifugation was used to isolate newly synthesized DNA from defined periods of S phase. The DNA was analyzed by Southern hybridization with a cloned probe containing one of the H4 histone genes of Physarum. The results indicate that the two H4 histone genes are replicated in the first 30 min of S phase but not exactly at the same time. H41 is replicated during the first 10 min of S phase, when only 15% of the genome is duplicated, whereas H42 replicates between 20 and 30 min after the onset of S phase. The possible relationship between the periodic expression of the genes and the timing of their replication is discussed.
在多头绒泡菌自然同步的有丝分裂周期中，测定了两个H4组蛋白基因（H41和H42）的复制时间。采用5-溴-2'-脱氧尿苷标记和密度梯度离心法，从S期的特定时间段分离新合成的DNA。用含有多头绒泡菌H4组蛋白基因之一的克隆探针通过Southern杂交分析DNA。结果表明，两个H4组蛋白基因在S期的前30分钟复制，但并非完全同时进行。H41在S期的前10分钟复制，此时基因组仅15%被复制，而H42在S期开始后20至30分钟之间复制。文中讨论了这些基因的周期性表达与其复制时间之间的可能关系。
Zotero 插件
