α和β珠蛋白DNA序列的复制发生在小鼠红白血病细胞的S期早期。
Replication of alpha and beta globin DNA sequences occurs during early S phase in murine erythroleukemia cells.
作者信息
Epner E, Rifkind R A, Marks P A
出版信息
Proc Natl Acad Sci U S A. 1981 May;78(5):3058-62. doi: 10.1073/pnas.78.5.3058.
Abstract
Murine erythroleukemia cells (MELC) can be induced to express the characteristics of erythroid differentiation by a variety of agents. Previous studies indicate that an action of inducer, occurring during early S phase, may be critical to the expression of differentiated characteristics such as initiation of accumulation of newly synthesized alpha and beta globin mRNAs. In this investigation, the time of replication of globin genes in MELC was studied. DNA was isolated from synchronous populations of cells obtained by centrifugal elutriation. Newly replicated DNA sequences were prepared from synchronized cells cultured for 1 1/2 hr with 5-bromodeoxyuridine; bromodeoxyuridine-containing DNA was isolated by CsCl gradient centrifugation. By employing cloned probes for hybridization to newly synthesized DNA, it was found that alpha and beta globin gene sequences are replicated early in S phase, while ribosomal RNA gene sequences are replicated to about the same extent in early, middle, and late S phases.
摘要
小鼠红白血病细胞（MELC）可被多种因子诱导表达红系分化特征。先前的研究表明，诱导剂在S期早期的作用可能对分化特征的表达至关重要，如新合成的α和β珠蛋白mRNA积累的起始。在本研究中，对MELC中珠蛋白基因的复制时间进行了研究。DNA从通过离心淘析获得的同步化细胞群体中分离出来。从用5-溴脱氧尿苷培养1.5小时的同步化细胞中制备新复制的DNA序列；含溴脱氧尿苷的DNA通过CsCl梯度离心分离。通过使用克隆探针与新合成的DNA杂交，发现α和β珠蛋白基因序列在S期早期复制，而核糖体RNA基因序列在S期早期、中期和晚期复制程度大致相同。
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Proc Natl Acad Sci U S A. 1981 May;78(5):3058-62. doi: 10.1073/pnas.78.5.3058.
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