Osley M A, Hereford L
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7689-93. doi: 10.1073/pnas.79.24.7689.
Sequences required for the regulated expression of a yeast histone 2A (H2A) gene have been investigated by using fusions between this gene and the Escherichia coli lacZ gene. Fusions containing the entire spacer region in which divergent transcription of the H2A and H2B genes is initiated result in low-level constitutive synthesis of beta-galactosidase (beta-D-galactoside galactohydrolase, EC 3.2.1.23) in yeast. Regulated expression (which is characterized by periodic synthesis during the S phase of the cell cycle) is restored when a 1.3-kilobase HindIII fragment containing a small region of the 3' end of the H2B gene is present in either orientation. The regulatory activity in this region appears to be coincident with a sequence that supports autonomous replication in yeast.
通过利用酵母组蛋白2A（H2A）基因与大肠杆菌lacZ基因之间的融合，对该酵母基因调控表达所需的序列进行了研究。含有整个间隔区的融合体（H2A和H2B基因的发散转录在此起始）导致酵母中β-半乳糖苷酶（β-D-半乳糖苷半乳糖水解酶，EC 3.2.1.23）的低水平组成型合成。当含有H2B基因3'端小区域的1.3千碱基HindIII片段以任何一种方向存在时，调控表达（其特征是在细胞周期的S期进行周期性合成）得以恢复。该区域的调控活性似乎与支持酵母自主复制的一个序列一致。
