Department of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
J Transl Med. 2024 Apr 17;22(1):364. doi: 10.1186/s12967-024-05167-x.
Polycystic ovary syndrome (PCOS) is an endocrinological and metabolic disorder that can lead to female infertility. Lipid metabolomics and proteomics are the new disciplines in systems biology aimed to discover metabolic pathway changes in diseases and diagnosis of biomarkers. This study aims to reveal the features of PCOS to explore its pathogenesis at the protein and metabolic level.
We collected follicular fluid samples and granulosa cells of women with PCOS and normal women who underwent in vitro fertilization(IVF) and embryo transfer were recruited. The samples were for the lipidomic study and the proteomic study based on the latest metabolomics and proteomics research platform.
Lipid metabolomic analysis revealed abnormal metabolism of glycerides, glycerophospholipids, and sphingomyelin in the FF of PCOS. Differential lipids were strongly linked with the rate of high-quality embryos. In total, 144 differentially expressed proteins were screened in ovarian granulosa cells in women with PCOS compared to controls. Go functional enrichment analysis showed that differential proteins were associated with blood coagulation and lead to follicular development disorders.
The results showed that the differential lipid metabolites and proteins in PCOS were closely related to follicle quality,which can be potential biomarkers for oocyte maturation and ART outcomes.
多囊卵巢综合征（PCOS）是一种内分泌和代谢紊乱的疾病，可能导致女性不孕。脂质组学和蛋白质组学是系统生物学中的新兴学科，旨在发现疾病中的代谢途径变化和诊断生物标志物。本研究旨在揭示 PCOS 的特征，探索其在蛋白质和代谢水平上的发病机制。
我们收集了接受体外受精（IVF）和胚胎移植的 PCOS 患者和正常女性的卵泡液样本和颗粒细胞。这些样本用于基于最新代谢组学和蛋白质组学研究平台的脂质组学研究和蛋白质组学研究。
脂质代谢组学分析显示 PCOS 的 FF 中甘油酯、甘油磷脂和鞘磷脂代谢异常。差异脂质与优质胚胎率密切相关。与对照组相比，PCOS 患者的卵巢颗粒细胞中筛选出 144 种差异表达蛋白。GO 功能富集分析表明，差异蛋白与血液凝固有关，导致卵泡发育障碍。
结果表明，PCOS 中的差异脂质代谢物和蛋白质与卵泡质量密切相关，它们可能是卵母细胞成熟和 ART 结果的潜在生物标志物。
