Human Reproduction Section, Division of Urology, Department of Surgery, Sao Paulo Federal University, Rua Embau, 231, São Paulo, SP, 04039-060, Brazil.
Laboratório Max Feffer de Genética de Plantas, Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, 13418-900, Brazil.
Metabolomics. 2018 Mar 16;14(4):51. doi: 10.1007/s11306-018-1350-z.
During in vitro fertilization (IVF), the hyper response to controlled ovarian stimulation (COS) is a common characteristic among patients diagnosed with polycystic ovary syndrome (PCOS), although non-diagnosed patients may also demonstrate this response.
In an effort to investigate follicular metabolic characteristics associated with hyper response to COS, the present study analyzed follicular fluid (FF) samples from patients undergoing IVF.
FF samples were obtained from patients with PCOS and hyper response during IVF (PCOS group, N = 15), patients without PCOS but with hyper response during IVF (HR group, N = 44), and normo-responder patients receiving IVF (control group, N = 22). FF samples underwent Bligh and Dyer extraction, followed by metabolomic analysis by ultra-performance liquid chromatography mass spectrometry, considering two technical replicates. Clinical data was analyzed by ANOVA and chi-square tests. The metabolomic dataset was analyzed by multivariate statistics, and the significance of biomarkers was confirmed by ANOVA.
Clinical data showed differences regarding follicles production, oocyte and embryo quality. From the 15 proposed biomarkers, 14 were of increased abundance in the control group and attributed as fatty acids, diacylglycerol, triacylglycerol, ceramide, ceramide-phosphate, phosphatidylcholine, and sphingomyelin. The PCOS patients showed increased abundance of a metabolite of m/z 144.0023 that was not attributed to a class.
The clinical and metabolic similarities observed in the FF of hyper responders with and without PCOS diagnosis indicate common biomarkers that could assist on the development of accessory tools for assessment of IVF parameters.
在体外受精（IVF）过程中，多囊卵巢综合征（PCOS）患者对控制性卵巢刺激（COS）的高反应是一种常见特征，尽管未被诊断出的患者也可能表现出这种反应。
为了研究与 COS 高反应相关的卵泡代谢特征，本研究分析了接受 IVF 的患者的卵泡液（FF）样本。
从接受 IVF 的 PCOS 患者和 IVF 期间高反应的患者（PCOS 组，N=15）、无 PCOS 但 IVF 期间高反应的患者（HR 组，N=44）和接受 IVF 的正常反应患者（对照组，N=22）中获取 FF 样本。FF 样本进行 Bligh 和 Dyer 提取，然后通过超高效液相色谱质谱进行代谢组学分析，考虑两个技术重复。通过方差分析和卡方检验分析临床数据。通过多变量统计分析代谢组数据集，并通过 ANOVA 验证生物标志物的显著性。
临床数据显示卵泡生成、卵母细胞和胚胎质量方面存在差异。从提出的 15 个生物标志物中，有 14 个在对照组中丰度增加，归因于脂肪酸、二酰基甘油、三酰基甘油、神经酰胺、神经酰胺磷酸、磷脂酰胆碱和鞘磷脂。PCOS 患者表现出 m/z 144.0023 的代谢物丰度增加，该代谢物未归因于某一类。
有和没有 PCOS 诊断的高反应者的 FF 中观察到的临床和代谢相似性表明存在共同的生物标志物，这可能有助于开发评估 IVF 参数的辅助工具。
