Wang Chen, Gu Bobo, Qi Shuhong, Hu Siyi, Wang Yu
Med-X Research Institute & School of Biomedical Engineering, Shanghai Jiao Tong University 1954 Huashan Road Shanghai 200030 China
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology Wuhan Hubei 430074 China.
Nanoscale Adv. 2024 Mar 5;6(8):2075-2087. doi: 10.1039/d4na00032c. eCollection 2024 Apr 16.
Phototherapy is a promising modality that could eradicate tumor and trigger immune responses immunogenic cell death (ICD) to enhance anti-tumor immunity. However, due to the lack of deep-tissue-excitable phototherapeutic agents and appropriate excitation strategies, the utility of phototherapy for efficient activation of the immune system is challenging. Herein, we report functionalized ICG nanoparticles (NPs) with the capture capability of tumor-associated antigens (TAAs). Under near-infrared (NIR) light excitation, the ICG NPs exhibited high-performance phototherapy, , synergistic photothermal therapy and photodynamic therapy, thereby efficiently eradicating primary solid tumor and inducing ICD and subsequently releasing TAAs. The ICG NPs also captured TAAs and delivered them to sentinel lymph nodes, and then the sentinel lymph nodes were activated with NIR light to trigger efficient T-cell immune responses through activation of dendritic cells with the assistance of ICG NP generated reactive oxygen species, inhibiting residual primary tumor recurrence and controlling distant tumor growth. The strategy of NIR light excited phototherapy in tumor sites and photo-activation in sentinel lymph nodes provides a powerful platform for active immune systems for anti-tumor photo-immunotherapy.
光疗是一种很有前景的治疗方式，它可以根除肿瘤并触发免疫反应——免疫原性细胞死亡（ICD），从而增强抗肿瘤免疫力。然而，由于缺乏可深层组织激发的光疗药物和合适的激发策略，光疗有效激活免疫系统的应用面临挑战。在此，我们报道了具有肿瘤相关抗原（TAA）捕获能力的功能化吲哚菁绿纳米颗粒（NP）。在近红外（NIR）光激发下，吲哚菁绿纳米颗粒展现出高性能光疗，即协同光热疗法和光动力疗法，从而有效根除原发性实体肿瘤并诱导ICD，随后释放TAA。吲哚菁绿纳米颗粒还捕获TAA并将其递送至前哨淋巴结，然后用近红外光激活前哨淋巴结，通过吲哚菁绿纳米颗粒产生的活性氧协助激活树突状细胞，触发有效的T细胞免疫反应，抑制原发性肿瘤残留复发并控制远处肿瘤生长。肿瘤部位近红外光激发光疗和前哨淋巴结光激活的策略为抗肿瘤光免疫治疗的主动免疫系统提供了一个强大平台。
