Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Faculty of Veterinary Medicine, Veterinary Hospital, Zagazig, Egypt.
Open Vet J. 2024 Jan;14(1):553-563. doi: 10.5455/OVJ.2024.v14.i1.50. Epub 2024 Jan 31.
Bacterial infections causing digestive problems are among the most serious threats to Egypt's duck industry, owing to their effects on feed utilization and body weight gain.
As a result, the goal of this study was to identify bacterial pathogens causing enteritis in ducks as well as testing their antimicrobials resistance capabilities.
Forty-two duck flocks from different localities at four Egyptian Governorates (El-Sharkia, El-Gharbia, El-Dakahlia, and El-Qaliobia) have been subjected to clinical and postmortem examination as well as bacterial isolation and identification. The liver samples have been collected aseptically from freshly euthanized ducks for bacterial isolation followed by identification using conventional biochemical tests, VITEK 2 system, and confirmatory polymerase chain reaction (PCR) for detection of the uid A gene (beta-glucuronidase enzyme) of In addition, antimicrobial sensitivity testing for the isolates against different antimicrobials by the VITEK 2 system was used.
Forty-six positive bacterial isolates were identified using conventional methods and the VITEK 2 system including spp. (52.17%), (41.30%), and 2.17% for each of , and . PCR was positive for uid A gene at 556 bp. The antibiogram patterns of isolated pathogens from naturally infected ducks in our work demonstrated 87% multidrug resistance with varying results against different antimicrobial drugs tested. Such findings supported the fact of the upgrading multidrug resistance of Staphylococci and Enterobacteriacae.
The most prevalent bacterial pathogens associated with duck enteritis were Staphylococcus spp. and with the first report of causing duck enteritis in Egypt.
导致消化问题的细菌感染是埃及养鸭业最严重的威胁之一，因为它们会影响饲料利用率和体重增加。
因此，本研究的目的是确定引起鸭肠炎的细菌病原体，并测试它们的抗菌药物耐药能力。
从埃及四个省（ Sharkia、Gharbia、Dakahlia 和 Qaliobia ）的不同地区的 42 个鸭群进行临床和剖检检查以及细菌分离和鉴定。从刚安乐死的鸭子无菌采集肝脏样本进行细菌分离，然后使用常规生化试验、VITEK 2 系统和 uid A 基因（β-葡萄糖醛酸酶酶）的聚合酶链反应（PCR）进行鉴定。此外，还使用 VITEK 2 系统对分离株进行了针对不同抗菌药物的药敏试验。
使用常规方法和 VITEK 2 系统鉴定出 46 株阳性细菌分离株，包括 spp.（52.17%）、 （41.30%）和 （各占 2.17%）。PCR 对 556 bp 处的 uid A 基因呈阳性。本工作中从自然感染鸭分离的病原体的药敏谱图显示 87%的多药耐药性，对不同测试抗菌药物的结果各不相同。这些发现支持葡萄球菌和肠杆菌科的多药耐药性升级的事实。
与鸭肠炎相关的最常见细菌病原体是葡萄球菌属和 ，这是首次报道 引起埃及鸭肠炎。
