Ammar Ahmed M, El-Naenaeey El-Sayed Y, El-Malt Rania M S, El-Gedawy Attia A, Khalifa Eman, Elnahriry Shimaa S, Abd El-Hamid Marwa I
Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
Department of Microbiology, Animal Health Research Institute, Zagazig 44516, Egypt.
Animals (Basel). 2020 Dec 22;11(1):3. doi: 10.3390/ani11010003.
is the leading cause of foodborne bacterial gastroenteritis in humans worldwide. Contaminated chickens and their products are the main sources of human campylobacteriosis. Therefore, this study aimed to detect the genotypic and virulence genes' profiles of multi-drug resistant (MDR) isolates and to assess the effects of sub-inhibitory concentrations (SICs) of eugenol and beta-resorcylic acid on the virulence of avian MDR isolates. These isolates were clustered together with the human isolates via enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) fingerprinting. A total of 345 samples were collected from human stool (100) and different chicken (245) samples in Sharkia Governorate, Egypt. Conventional phenotypic methods identified 113 isolates (32.8%) as and all isolates were MDR and resistant to erythromycin and ampicillin. The genes B11, N, and A were detected in 52%, 36% and 100% strains, respectively. ERIC-PCR yielded 14 profiles and five main clusters. Interestingly, human and chicken isolates were clustered together in ERIC-PCR clusters II-V, which confirmed the genetic relatedness between the isolates from both origins. Beta-resorcylic acid and eugenol inhibited the invasion of isolates to chicken intestinal cells by 41.66-38.19% and 31.94-29.16%, respectively, and minimized the transcription of A, B11, and N genes in the tested isolates by real-time quantitative reverse transcription PCR (qRT-PCR). In essence, eugenol and beta-resorcylic acid are promising natural antimicrobials for minimizing the virulence of MDR in chickens, thereby managing human campylobacteriosis.
是全球人类食源性细菌性肠胃炎的主要病因。受污染的鸡及其产品是人类弯曲杆菌病的主要来源。因此，本研究旨在检测多重耐药（MDR）分离株的基因型和毒力基因谱，并评估丁香酚和β-间苯二酚亚抑菌浓度（SICs）对禽MDR分离株毒力的影响。通过肠杆菌重复基因间共识PCR（ERIC-PCR）指纹图谱分析，这些分离株与人类分离株聚集在一起。在埃及谢赫村省，共采集了345份样本，其中包括100份人类粪便样本和245份不同的鸡肉样本。传统表型方法鉴定出113株分离株（32.8%）为[此处原文缺失相关细菌名称]，所有分离株均为多重耐药，且对红霉素和氨苄青霉素耐药。分别在52%、36%和100%的菌株中检测到基因B11、N和A。ERIC-PCR产生了14种图谱和5个主要聚类。有趣的是，人类和鸡肉[此处原文缺失相关细菌名称]分离株在ERIC-PCR聚类II-V中聚集在一起，这证实了来自两个来源的分离株之间的遗传相关性。β-间苯二酚和丁香酚分别抑制[此处原文缺失相关细菌名称]分离株对鸡肠细胞的侵袭41.66 - 38.19%和31.94 - 29.16%，并通过实时定量逆转录PCR（qRT-PCR）使受试分离株中A、B11和N基因的转录最小化。从本质上讲，丁香酚和β-间苯二酚是有前景的天然抗菌剂，可降低鸡中多重耐药[此处原文缺失相关细菌名称]的毒力，从而控制人类弯曲杆菌病。
