Yun Lisa, Sakkos Jonathan K, Ducat Daniel C
DOE-MSU Plant Research Laboratories, Michigan State University, East Lansing, Michigan, United States.
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan, United States.
MicroPubl Biol. 2024 Apr 2;2024. doi: 10.17912/micropub.biology.001097. eCollection 2024.
Surface display technologies have been primarily developed for heterotrophic microbes, leaving photosynthetic counterparts like cyanobacteria with limited molecular tools. Here, we expanded upon surface display systems in PCC 7942 by modifying two outer-membrane proteins, SomA and Intimin, to display tags ( , SpyTag) to mediate physical interactions of living cyanobacteria with other biotic and abiotic targets. While re-engineered SomA constructs successfully translocated to the cell surface and could bind to compatible ligands, the efficacy of the best-performing designs was limited by a poorly-understood heterogeneity in the accessibility of the tags in living cells, resulting in low attachment penetrance.
表面展示技术主要是为异养微生物开发的，使得像蓝细菌这样的光合微生物可用的分子工具有限。在这里，我们通过修饰两种外膜蛋白SomA和intimin，在PCC 7942中扩展了表面展示系统，以展示标签（如SpyTag），从而介导活蓝细菌与其他生物和非生物靶标的物理相互作用。虽然重新设计的SomA构建体成功转运到细胞表面并能与兼容的配体结合，但最佳设计的效果受到活细胞中标签可及性方面一个尚未充分理解的异质性的限制，导致附着渗透率较低。
