Assam Science and Technology University, Jalukbari, Guwahati, Assam, India.
School of Pharmaceutical Science, Girijananda Chowdhury University, Guwahati, Assam, India.
J Vector Borne Dis. 2024 Jul 1;61(3):435-443. doi: 10.4103/JVBD.JVBD_181_23. Epub 2024 Sep 21.
At present, use of synthetic pesticides to manage insects and other arthropods creates a number of issues that are related to the environment and public health. The goal of the present study is to find repellent activity of Vitex negundo and Curcuma longa herbal extracts against the wild species Simulium (blackfly) bite.
We have studied Simulium biting behavior and tested the repellency of herbal extract obtained from Vitex negundo L. (Lamiales: Lamiaceae) and Curcuma longa L. (Zingiberal: Zingiberaceae) along with their essential oils in three locations in Arunachal Pradesh, India on human volunteers' resistance to Simulium (blackflies). The reported herbal extracts were incorporated to topical drug delivery vehicle as a cream and gel.
The methanolic extract of Vitex negundo cream and gel formulation showed >2 h safety at 5% concentration and >5 h safety at 10% concentration in all the testing sites followed by ethyl acetate extract. Whereas, chloroform extract of Curcuma longa cream and gel formulation provided >1 h safety at 5% concentration and >4 h safety at 10% concentration.
At an optimum concentration of 10%, the methanolic extract of Vitex negundo cream provided complete protection times (CPTs) 320.4, 358.6, and 346.4 min, respectively. These findings offer confirmation of the methanolic extract and chloroform extract potential for use in new blackfly repellents.
目前，使用合成农药来防治昆虫和其他节肢动物会产生一些与环境和公共卫生有关的问题。本研究的目的是寻找荆芥和姜黄的植物提取物对野生斯氏按蚊（黑蝇）叮咬的驱避活性。
我们研究了斯氏按蚊的叮咬行为，并在印度阿鲁纳恰尔邦的三个地点测试了荆芥（唇形科：马鞭草科）和姜黄（姜科：姜科）的植物提取物及其精油对人体志愿者抵抗斯氏按蚊（黑蝇）的驱避作用。报道的草药提取物被纳入局部药物输送载体，制成乳膏和凝胶。
荆芥乳膏和凝胶制剂的甲醇提取物在所有测试地点的 5%浓度下具有超过 2 小时的安全性，在 10%浓度下具有超过 5 小时的安全性，其次是乙酸乙酯提取物。而姜黄乳膏和凝胶制剂的氯仿提取物在 5%浓度下提供了超过 1 小时的安全性，在 10%浓度下提供了超过 4 小时的安全性。
在最佳浓度 10%下，荆芥乳膏的甲醇提取物分别提供了 320.4、358.6 和 346.4 分钟的完全保护时间（CPT）。这些发现证实了荆芥和姜黄甲醇提取物和氯仿提取物具有作为新型黑蝇驱避剂的潜力。
