Department of Clinical Laboratory, Affiliated Hospital of Panzhihua University, Panzhihua, China.
Lupus. 2024 Jun;33(7):675-684. doi: 10.1177/09612033241243175. Epub 2024 Apr 18.
This study aimed to investigate the expression of H19 and its possible molecular mechanism in systemic lupus erythematosus (SLE).
The expression of H19 and miR-19b in serum and peripheral blood mononuclear cells (PBMCs) were detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Receiver operator characteristic (ROC) curve was constructed to evaluate the diagnostic value of serum H19 in SLE. Pearson correlation coefficient was used to analyze the correlation between serum levels of H19 and miR-19b. Flow cytometry and Cell counting kit-8 (CCK-8) assay were performed to detect cell apoptosis and viability. The levels of pro-inflammatory and anti-inflammatory factors were measured by enzyme-linked immunosorbent assay (ELISA). Luciferase reporter gene assay was conducted to verify the interaction between H19 and miR-19b.
The expression of H19 and miR-19b in SLE group were up-regulated and down-regulated, respectively. Serum H19 has certain clinical diagnostic value in SLE. In in vitro studies, overexpression of H19 can significantly inhibit the viability of PBMCs and promote apoptosis and inflammatory response of PBMCs by interacting with miR-19b.
The expression of H19 is upregulated in patients with SLE and plays a role in cell function and inflammation by targeting miR-19b in PBMCs, which may be one of the pathological mechanisms of SLE.
本研究旨在探讨 H19 在系统性红斑狼疮（SLE）中的表达及其可能的分子机制。
采用逆转录定量聚合酶链反应（RT-qPCR）检测血清和外周血单个核细胞（PBMCs）中 H19 和 miR-19b 的表达。绘制受试者工作特征（ROC）曲线评估血清 H19 在 SLE 中的诊断价值。采用 Pearson 相关系数分析血清 H19 水平与 miR-19b 的相关性。采用流式细胞术和细胞计数试剂盒-8（CCK-8）检测细胞凋亡和活力。采用酶联免疫吸附试验（ELISA）测定促炎和抗炎因子水平。通过荧光素酶报告基因实验验证 H19 与 miR-19b 的相互作用。
SLE 组 H19 和 miR-19b 的表达均上调和下调。血清 H19 在 SLE 中有一定的临床诊断价值。在体外研究中，过表达 H19 可通过与 miR-19b 相互作用，显著抑制 PBMCs 的活力，并促进其凋亡和炎症反应。
H19 在 SLE 患者中表达上调，通过靶向 PBMCs 中的 miR-19b 发挥作用，影响细胞功能和炎症，这可能是 SLE 的病理机制之一。
