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miR-183-5p 是系统性红斑狼疮的潜在分子标志物。

miR-183-5p Is a Potential Molecular Marker of Systemic Lupus Erythematosus.

机构信息

Department of Medical Genetics and Cell Biology, College of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia, China.

Department of Rheumatology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.

出版信息

J Immunol Res. 2021 May 6;2021:5547635. doi: 10.1155/2021/5547635. eCollection 2021.

DOI:10.1155/2021/5547635
PMID:34036107
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8124875/
Abstract

OBJECTIVE

To investigate microRNA (miRNA) expression profiles in individuals with systemic lupus erythematosus (SLE) and identify the valuable miRNA biomarkers in diagnosing and monitoring SLE.

METHODS

Next-generation sequencing (NGS) was performed to assess miRNA amounts in peripheral blood mononuclear cells (PBMCs) from four SLE cases and four healthy controls. Quantitative polymerase chain reaction (qPCR) was carried out for validating candidate miRNAs in 32 SLE cases and 32 healthy controls. In addition, receiver operating characteristic (ROC) curve analysis was completed to evaluate diagnostic performance. Finally, the associations of candidate miRNAs with various characteristics of SLE were analyzed.

RESULTS

A total of 157 miRNAs were upregulated, and 110 miRNAs were downregulated in PBMCs from SLE cases in comparison to healthy controls, of which the increase of miR-183-5p and decrease of miR-374b-3p were validated by qPCR and both showed good diagnostic performance for SLE diagnosis. Besides, miR-183-5p expression levels displayed a positive association with SLE disease activity index (SLEDAI) and anti-dsDNA antibody amounts.

CONCLUSION

Our data indicated that miR-183-5p is a promising biomarker of SLE.

摘要

目的

研究系统性红斑狼疮（SLE）患者的微小 RNA（miRNA）表达谱，鉴定用于诊断和监测 SLE 的有价值的 miRNA 生物标志物。

方法

采用下一代测序（NGS）技术评估 4 例 SLE 病例和 4 例健康对照者外周血单个核细胞（PBMC）中的 miRNA 含量。采用定量聚合酶链反应（qPCR）对 32 例 SLE 病例和 32 例健康对照者中的候选 miRNA 进行验证。此外，还进行了受试者工作特征（ROC）曲线分析以评估诊断性能。最后，分析候选 miRNA 与 SLE 各种特征的相关性。

结果

与健康对照者相比，SLE 患者 PBMC 中共有 157 个 miRNA 上调，110 个 miRNA 下调，其中 miR-183-5p 的上调和 miR-374b-3p 的下调通过 qPCR 得到验证，两者均显示出良好的 SLE 诊断性能。此外，miR-183-5p 的表达水平与 SLE 疾病活动指数（SLEDAI）和抗 dsDNA 抗体量呈正相关。

结论

我们的数据表明，miR-183-5p 是 SLE 的一种有前途的生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/c8121d067fd6/JIR2021-5547635.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/3e4ba361b006/JIR2021-5547635.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/6ea25288d94a/JIR2021-5547635.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/210de2916677/JIR2021-5547635.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/29ff3569a75d/JIR2021-5547635.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/7a580049e947/JIR2021-5547635.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/d1b3a4fd7dfd/JIR2021-5547635.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/435e5b25c5e5/JIR2021-5547635.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/fdef1cb8d76b/JIR2021-5547635.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/c8121d067fd6/JIR2021-5547635.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/3e4ba361b006/JIR2021-5547635.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/6ea25288d94a/JIR2021-5547635.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/210de2916677/JIR2021-5547635.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/29ff3569a75d/JIR2021-5547635.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/7a580049e947/JIR2021-5547635.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/d1b3a4fd7dfd/JIR2021-5547635.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/435e5b25c5e5/JIR2021-5547635.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/fdef1cb8d76b/JIR2021-5547635.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9866/8124875/c8121d067fd6/JIR2021-5547635.009.jpg

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