Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito Exterior s/n. Coyoacán, 04510, Ciudad de Mexico, Mexico.
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito Exterior s/n. Coyoacán, 04510, Ciudad de Mexico, Mexico.
Chem Commun (Camb). 2024 May 7;60(38):5062-5065. doi: 10.1039/d4cc01144a.
This study introduces a paradigm-shifting approach to optimize mitochondrial targeting. Employing a new fluorescent probe strategy, we unravel a combined influence of both Nernst potential () and partitioning () contributions. Through the synthesis of new benz[]indolinium-derived probes, our findings redefine the landscape of mitochondrial localization by optimizing the efficacy of mitochondrial probe retention in primary cortical neurons undergoing normoxia and oxygen-glucose deprivation. This methodology not only advances our understanding of subcellular dynamics, but also holds promise for transformative applications in biomedical research and therapeutic development.
本研究提出了一种颠覆性的方法来优化线粒体靶向。采用新的荧光探针策略，我们揭示了 Nernst 势（）和分配（）贡献的综合影响。通过合成新的苯并[]吲哚啉衍生探针，我们的发现通过优化在经历正常氧和氧葡萄糖剥夺的原代皮质神经元中保留线粒体探针的功效，重新定义了线粒体定位的格局。这种方法不仅推进了我们对亚细胞动力学的理解，而且在生物医学研究和治疗开发方面具有变革性的应用前景。
