Department of Ecology, School of Resources and Environmental Engineering, Anhui University, Hefei, 230601, China.
Key Laboratory of Genetic Evolution & Animal Models, Kunming Institute of Zoology, Chinese Academy of Sciences (CAS), Kunming, Yunnan, 650223, China.
Sci Data. 2024 Apr 18;11(1):396. doi: 10.1038/s41597-024-03251-x.
Stag beetles (Coleoptera: Lucanidae) represent a significant saproxylic assemblage in forest ecosystems and are noted for their enlarged mandibles and male polymorphism. Despite their relevance as ideal models for the study of exaggerated mandibles that aid in attracting mates, the regulatory mechanisms associated with these traits remain understudied, and restricted by the lack of high-quality reference genomes for stag beetles. To address this limitation, we successfully assembled the first chromosome-level genome of a representative species Dorcus hopei. The genome was 496.58 Mb in length, with a scaffold N50 size of 54.61 Mb, BUSCO values of 99.8%, and 96.8% of scaffolds anchored to nine pairs of chromosomes. We identified 285.27 Mb (57.45%) of repeat sequences and annotated 11,231 protein-coding genes. This genome will be a valuable resource for further understanding the evolution and ecology of stag beetles, and provides a basis for studying the mechanisms of exaggerated mandibles through comparative analysis.
鹿角甲虫（鞘翅目：鹿角甲科）是森林生态系统中重要的腐木生物群落，以其巨大的下颚和雄性多态性而闻名。尽管它们是研究有助于吸引配偶的夸张下颚的理想模型，但与这些特征相关的调节机制仍未得到充分研究，并且受到鹿角甲虫缺乏高质量参考基因组的限制。为了解决这一限制，我们成功组装了代表性物种冀鹿锹甲 Dorcus hopei 的第一个染色体水平基因组。该基因组长 496.58Mb，支架 N50 大小为 54.61Mb，BUSCO 值为 99.8%，96.8%的支架锚定在九对染色体上。我们鉴定了 285.27Mb（57.45%）的重复序列和注释了 11,231 个蛋白质编码基因。这个基因组将是进一步了解鹿角甲虫进化和生态学的宝贵资源，并为通过比较分析研究夸张下颚的机制提供了基础。
