1Helmholtz International Lab for Anti-Infectives, State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao, Shandong, China.
2School of Life Sciences, Shandong University, Qingdao, Shandong, China.
Annu Rev Anal Chem (Palo Alto Calif). 2024 Jul;17(1):265-288. doi: 10.1146/annurev-anchem-061622-034229. Epub 2024 Jul 2.
Bioluminescence imaging (BLI) is a powerful method for visualizing biological processes and tracking cells. Engineered bioluminescent bacteria that utilize luciferase-catalyzed biochemical reactions to generate luminescence have become useful analytical tools for in vitro and in vivo bacterial imaging. Accordingly, this review initially introduces the development of engineered bioluminescent bacteria that use different luciferase-luciferin pairs as analytical tools and their applications for in vivo BLI, including real-time bacterial tracking of infection, probiotic investigation, tumor-targeted therapy, and drug screening. Applications of engineered bioluminescent bacteria as whole-cell biosensors for sensing biological changes in vitro and in vivo are then discussed. Finally, we review the optimizations and future directions of bioluminescent bacteria for imaging. This review aims to provide fundamental insights into bacterial BLI and highlight the potential development of this technique in the future.
生物发光成像是一种可视化生物过程和追踪细胞的强大方法。利用荧光素酶催化的生化反应产生发光的工程化生物发光细菌已成为体外和体内细菌成像的有用分析工具。因此，本综述首先介绍了利用不同荧光素酶-荧光素对作为分析工具的工程化生物发光细菌的发展及其在体内生物发光成像中的应用，包括感染的实时细菌追踪、益生菌研究、肿瘤靶向治疗和药物筛选。然后讨论了工程化生物发光细菌作为体外和体内生物变化的全细胞生物传感器的应用。最后，我们综述了生物发光细菌成像的优化和未来方向。本综述旨在为细菌生物发光成像提供基本的见解，并强调该技术未来的潜在发展。
