Molecular Functional Imaging Lab, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Sector 22, Kharghar, Navi Mumbai 410210, Maharashtra, India.
Curr Opin Pharmacol. 2012 Oct;12(5):592-600. doi: 10.1016/j.coph.2012.07.014. Epub 2012 Sep 3.
Bioluminescence is the biologically active luminescence light producing event encountered in nature. In recent years several new screening methods utilizing bioluminescent cell-based biosensors have been designed demonstrating their utility towards dynamic monitoring of a variety of cellular functions. Because luciferase is unnatural to mammalian physiology, assays utilizing specific substrates to yield a luminescent signal are attractive and serve the purpose with high sensitivity and specificity. Often genetic or chemical modifications in different luciferase-substrate system in use have afforded new functionalities making these assays even more robust. Finally, in the evolving paradigm of molecular imaging, in vivo bioluminescence imaging (BLI) has evolved as a very attractive tool for interrogating human cellular biology in rodent models. In this short review we explore various bioluminescence screening strategies developed and analyze their scope in future drug screening processes.
生物发光是自然界中存在的生物活性发光现象。近年来，已经设计了几种利用生物发光细胞基生物传感器的新筛选方法，证明了它们在动态监测各种细胞功能方面的实用性。由于荧光素酶对哺乳动物的生理是不自然的，因此利用特定底物产生发光信号的测定方法具有吸引力，并具有高灵敏度和特异性。通常，在使用的不同荧光素酶-底物系统中的遗传或化学修饰提供了新的功能，使这些测定方法更加稳健。最后，在分子成像的不断发展的范例中，体内生物发光成像（BLI）已成为在啮齿动物模型中研究人类细胞生物学的非常有吸引力的工具。在这篇简短的综述中，我们探讨了已开发的各种生物发光筛选策略，并分析了它们在未来药物筛选过程中的应用范围。
