载细胞外囊泡的丝素蛋白和羟丙基纤维素复合可注射水凝胶用于心肌梗死修复。

Silk fibroin and hydroxypropyl cellulose composite injectable hydrogel-containing extracellular vesicles for myocardial infarction repair.

机构信息

School of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou, 310018, People's Republic of China.

International Scientific and Technological Cooperation Base of Intelligent Biomaterials and Functional Fibers of Zhejiang Province, Hangzhou, 310018, People's Republic of China.

出版信息

Biomed Phys Eng Express. 2024 Apr 30;10(4). doi: 10.1088/2057-1976/ad40b2.

DOI:10.1088/2057-1976/ad40b2

PMID:38640908

Abstract

Extracellular vesicles (EVs) have been recognized as one of the promising specific drugs for myocardial infarction (MI) prognosis. Nevertheless, low intramyocardial retention of EVs remains a major impediment to their clinical application. In this study, we developed a silk fibroin/hydroxypropyl cellulose (SF/HPC) composite hydrogel combined with AC16 cell-derived EVs targeted modification by folic acid for the treatment of acute myocardial infarction repair. EVs were functionalized by distearoylphosphatidyl ethanolamine-polyethylene glycol (DSPE-PEG-FA) via noncovalent interaction for targeting and accelerating myocardial infarction repair., cytocompatibility analyses revealed that the as-prepared hydrogels had excellent cell viability by MTT assay and the functionalized EVs had higher cell migration by scratch assay., the composite hydrogels can promote myocardial tissue repair effects by delaying the process of myocardial fibrosis and promoting angiogenesis of infarct area in MI rat model.

摘要

细胞外囊泡 (EVs) 已被认为是一种有前途的治疗心肌梗死 (MI) 的特异性药物。然而，EVs 在心肌内的滞留率低仍是其临床应用的主要障碍。在本研究中，我们开发了一种丝素蛋白/羟丙基纤维素 (SF/HPC) 复合水凝胶，该水凝胶结合了叶酸靶向修饰的 AC16 细胞来源的 EVs，用于治疗急性心肌梗死修复。通过非共价相互作用将 EVs 功能化，得到二硬脂酰磷脂酰乙醇胺-聚乙二醇 (DSPE-PEG-FA)，以实现靶向和加速心肌梗死修复。细胞相容性分析表明，通过 MTT 分析，所制备的水凝胶具有优异的细胞活力，通过划痕试验，功能化的 EVs 具有更高的细胞迁移能力。在 MI 大鼠模型中，复合水凝胶通过延缓心肌纤维化过程和促进梗死区血管生成，可促进心肌组织修复效果。