Pharmaceutical Sciences and Technology Program, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
Center of Excellence in Natural Products and Nanoparticles (NP2), Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
Sci Rep. 2024 Apr 19;14(1):9018. doi: 10.1038/s41598-024-59357-0.
Cyperus rotundus rhizomes have been used in longevity remedies in Thailand for nourishing good health, which led us to investigate the effect on energy homeostasis, especially glucose utilization in myotubes and adipocytes, and on inhibition of lipogenesis in adipocytes. The results showed that an ethyl acetate extract of C. rotundus rhizomes (ECR) containing 1.61%w/w piceatannol, with a half-maximal concentration of 17.76 ± 0.03 μg/mL in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, caused upregulation and cell-membrane translocation of glucose transporters GLUT4 and 1 in L6 myotubes but downregulation and cytoplasmic localization of GLUT4 expression in 3T3-L1 adipocytes and was related to the p-Akt/Akt ratio in both cells, especially at 100 μg/mL. Moreover, ECR (25-100 μg/mL) significantly inhibited lipid accumulation via Adenosine Monophosphate-Activated Protein Kinase (AMPK), Acetyl CoA Carboxylase (ACC), and Glycogen Synthase Kinase (GSK) pathways. Its immunoblot showed increased expression of p-AMPKα/AMPKα and p-ACC/ACC but decreased expression of p-Akt/Akt and p-GSK3β/GSK3β in 3T3-L1 adipocytes. Moreover, the decreased expression of the adipogenic effectors, perilipin1 and lipoprotein lipase, in ECR-incubated adipocytes (50 and 100 μg/mL) indicated reduced de novo lipogenesis. Our study elucidated mechanisms of C. rotundus that help attenuate glucose tolerance in skeletal muscle and inhibit lipid droplet accumulation in adipose tissue.
香附根茎在泰国被用于延年益寿的补品，以滋养健康，这促使我们研究其对能量平衡的影响，特别是肌管和脂肪细胞中的葡萄糖利用，以及对脂肪细胞中脂肪生成的抑制作用。结果表明，香附根茎的乙酸乙酯提取物（ECR）含有 1.61%w/w 的白藜芦醇，在 2,2-二苯基-1-苦基肼（DPPH）自由基清除试验中，其半最大浓度为 17.76±0.03μg/mL，导致 L6 肌管中葡萄糖转运蛋白 GLUT4 和 1 的上调和细胞膜易位，但在 3T3-L1 脂肪细胞中 GLUT4 表达下调和细胞质定位，与两种细胞中的 p-Akt/Akt 比值有关，尤其是在 100μg/mL 时。此外，ECR（25-100μg/mL）通过腺苷单磷酸激活蛋白激酶（AMPK）、乙酰辅酶 A 羧化酶（ACC）和糖原合酶激酶（GSK）途径显著抑制脂质积累。其免疫印迹显示，在 3T3-L1 脂肪细胞中，p-AMPKα/AMPKα 和 p-ACC/ACC 的表达增加，而 p-Akt/Akt 和 p-GSK3β/GSK3β 的表达减少。此外，在 ECR 孵育的脂肪细胞（50 和 100μg/mL）中，脂肪生成效应物 perilipin1 和脂蛋白脂肪酶的表达减少表明从头脂肪生成减少。我们的研究阐明了香附有助于减轻骨骼肌葡萄糖耐量和抑制脂肪组织中脂肪滴积累的机制。
