Osaka Aquarium Kaiyukan, Osaka, Japan.
Laboratory of Veterinary Internal Medicine, Department of Veterinary Science, Osaka Metropolitan University, Osaka, Japan.
J Aquat Anim Health. 2024 Sep;36(3):239-249. doi: 10.1002/aah.10219. Epub 2024 Apr 20.
The use of computed tomography (CT) in aquarium animals, including elasmobranchs, has increased dramatically. To take advantage of CT, contrast medium is used to enhance internal organs and provide contrast since elasmobranchs lack visceral fat. In this study, the contrast effects of iopamidol were examined for up to 260 days after intravenous administration to establish the time course of the CT values for the target organs in eight mature Cloudy Catsharks Scyliorhinus torazame.
A micro-CT system was used to measure the CT values of the designated region of interest in the target organs (ventricular cavity, kidneys, liver, gallbladder, ovarian follicles, uterine horn cavity) over time and the eggs laid, following administration of iopamidol (700 mg of iodine/kg).
The CT values of the ventricular cavity and kidneys peaked at 30 min and showed low values after day 22. The CT values for the liver increased over time and peaked at day 200, whereas values for the gallbladder and ovarian follicles peaked on day 6, with the gallbladder showing a low value and the ovarian follicles still showing a high value on day 260. Computed tomography images with identifiable enhancement within bilateral uterine horns were followed from days 1 to 35. The mean and maximum CT values of yolk and jelly in eggs laid after day 30 were significantly higher than the values for eggs laid up to day 29; embryonic development was confirmed in 88.7% of the eggs.
There was no mortality or morbidity of the sharks during the experiment, indicating that the administration of iopamidol at 700 mg of iodine/kg did not result in any adverse effects for 260 days. This is the first study to describe the long-term contrast effects of iopamidol, thus contributing new information about the application of contrast studies in Cloudy Catsharks.
包括鲨鱼在内的水族馆动物的计算机断层扫描（CT）使用量急剧增加。为了利用 CT，使用对比剂来增强内部器官并提供对比，因为鲨鱼缺乏内脏脂肪。在这项研究中，检查了碘帕醇的对比效果，最长可达静脉注射后 260 天，以确定目标器官的 CT 值时间过程在 8 只成熟的云纹猫鲨 Scyliorhinus torazame 中。
使用微 CT 系统测量指定目标器官（心室腔、肾脏、肝脏、胆囊、卵巢滤泡、子宫角腔）的感兴趣区域的 CT 值，同时测量注射碘帕醇（700 毫克碘/千克）后产下的卵。
心室腔和肾脏的 CT 值在 30 分钟时达到峰值，在第 22 天之后呈现低值。肝脏的 CT 值随时间增加并在第 200 天达到峰值，而胆囊和卵巢滤泡的 CT 值在第 6 天达到峰值，胆囊呈低值，卵巢滤泡在第 260 天仍呈高值。双侧子宫角内可识别增强的 CT 图像从第 1 天到第 35 天进行了跟踪。第 30 天后产下的卵的卵黄和果冻的平均和最大 CT 值明显高于第 29 天之前产下的卵；88.7%的卵证实了胚胎发育。
在实验过程中，鲨鱼没有死亡或患病，这表明在 260 天内，以 700 毫克碘/千克的剂量给予碘帕醇不会产生任何不良反应。这是首次描述碘帕醇的长期对比效果的研究，因此为云纹猫鲨对比研究的应用提供了新的信息。
