Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical Research), 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.
Cytotechnology. 2000 Jul;33(1-3):259-64. doi: 10.1023/A:1008137817944.
Four analogs of succinoyl trehalose lipid-3 (STL-3)with saturated even-number or odd-number carbonchains, and unsaturated or halogenated fatty acidswere examined for their ability to inhibit the growthand induce the differentiation of HL-60 humanpromyelocytic leukemia cells. The optimalconcentration of STL-3 at which such activities wererecognized was closed to the critical micelleconcentration of STL-3. Analog of STL-3 witheven-number or odd-number carbon chain and unsaturatedfatty acids strongly inhibited growth and induced thedifferentiation of HL-60 cells, as evaluated in termsof nitroblue tetrazilium-reducing activity and theappearance of the CD36 antigen. An analog of STL-3with halogenated fatty acids significantly inhibitedproliferation but only induced the differentiation ofHL-60 cells. Our results indicate that the effects ofSTL-3 and its analogs on HL-60 cells depend on thestructure of the hydrophobic moiety of STL-3.
四种琥珀酰基海藻糖脂质-3（STL-3）类似物，其脂肪酸链具有饱和偶数或奇数碳链，以及不饱和或卤代脂肪酸，被研究其抑制 HL-60 人早幼粒细胞白血病细胞生长和诱导分化的能力。识别这些活性的最佳 STL-3 浓度接近 STL-3 的临界胶束浓度。具有偶数或奇数碳链和不饱和脂肪酸的 STL-3 类似物强烈抑制 HL-60 细胞的生长并诱导其分化，如通过硝基蓝四唑还原活性和 CD36 抗原的出现来评估。具有卤代脂肪酸的 STL-3 类似物显著抑制增殖，但仅诱导 HL-60 细胞分化。我们的结果表明，STL-3 及其类似物对 HL-60 细胞的作用取决于 STL-3 疏水区的结构。
